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Efficient inactivation of pseudotyped HIV-based lentiviral vectors and infectious HIV.
Journal of Virological Methods ( IF 3.1 ) Pub Date : 2019-11-05 , DOI: 10.1016/j.jviromet.2019.113768
Fabian Kriesel 1 , Lena Stegmann 2 , Sandra Ciesek 2 , Lars Kaderali 3 , Hanna-Mari Baldauf 1
Affiliation  

Lentiviral vectors and lentiviruses are important tools for basic and applied biomedical research. Yet, biosafety regulations from legal authorities have to be fulfilled when transferring BSL-2 to -3 vectors/viruses to facilities with lower biosafety level. Here, we (re-)evaluated different chemical and thermal approaches to inactivate vesicular stomatitis virus G-protein (VSV-G) pseudotyped lentiviral vectors and either wildtype or VSV-G pseudotyped human immunodeficiency viruses (HIV). Aldehydes, detergents and alcohols were as effective as thermal inactivation procedures to efficiently inactivate purified lentiviral vectors and replication-competent HIV. In addition, no residual infectivity was detected when inactivating HIV-infected TZM-bl reporter cells with selected detergents and aldehydes. Thus, our established inactivation protocols can be used by other laboratories working with lentiviral vectors or infectious lentiviruses and provide a template for viruses with similar physicochemical properties.

中文翻译:

高效灭活基于假型HIV的慢病毒载体和感染性HIV。

慢病毒载体和慢病毒是基础和应用生物医学研究的重要工具。但是,将BSL-2转换为-3载体/病毒到生物安全水平较低的设施时,必须遵守法律机构的生物安全规定。在这里,我们(重新)评估了灭活水泡性口炎病毒G蛋白(VSV-G)假型慢病毒载体和野生型或VSV-G假人免疫缺陷病毒(HIV)的不同化学和热学方法。醛,去污剂和醇与热灭活程序一样有效,可以有效地灭活纯化的慢病毒载体和具有复制能力的HIV。另外,当用选定的去污剂和醛灭活HIV感染的TZM-b1报告基因细胞时,没有检测到残留的感染性。从而,
更新日期:2019-11-01
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