The present study was conducted to determine the prevalence of C. burnetii in raw milk samples collected from water buffalos and cattle in Northwest of Iran (West Azerbaijan Province). A total number of 840 milk samples were randomly collected from buffalos and cattle belonged to three different geographical regions in west Azerbaijan (the map is necessary). The milk samples were collected seasonally during 2018 and the age of animals were recorded. All the milk samples were subjected to DNA extraction. Nested-PCR was used to detect C. burnetii based on the transposable gene IS1111. The results showed that 16.9% (95% CI: 14.5%-19.6%) of the examined milk samples (19.3% buffalo and 14.6% cattle samples) were positive for C. burnetii. There was a significant difference in C. burnetii shedding in milk between different age groups in cattle but not in buffalos (p value <0.05). The shedding of C. burnetii in milk was highly prevalent in summer (31.1%) (p < 0.05, 95% CI: 26.1%-38.4%). There were significant regional and seasonal variations in the prevalence of C. burnetii in the examined milk samples. It was concluded that buffalo population in west Azerbaijan should be considered as an important factor in the epidemiology of Q fever and consequently in public health.

中文翻译：

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从伊朗西北水牛和奶牛场收集的牛奶中，伯氏杆菌（Coxiella burnetii）的患病率。

进行本研究是为了确定伊朗西北部（西阿塞拜疆省）从水牛和牛中采集的生乳样品中的伯氏梭状芽胞杆菌的患病率。从阿塞拜疆西部三个不同地理区域的水牛和牛中随机采集了总共840个牛奶样品（有必要提供地图）。在2018年期间按季节收集了牛奶样品，并记录了动物的年龄。对所有牛奶样品进行DNA提取。基于转座基因IS1111，巢式PCR用于检测伯氏梭菌。结果表明，在所检查的牛奶样品（19.3％的水牛和14.6％的牛样品）中，有16.9％（95％CI：14.5％-19.6％）的伯氏梭菌呈阳性。C有显着差异。牛的不同年龄组之间的牛奶中的伯氏菌脱落，而水牛中的则不存在（p值<0.05）。夏季，牛奶中伯氏梭菌的脱落非常普遍（31.1％）（p <0.05，95％CI：26.1％-38.4％）。在检查的牛奶样本中，伯氏梭菌的流行存在明显的区域和季节变化。结论是，阿塞拜疆西部的水牛种群应被认为是Q热流行病学的重要因素，因此也是公共卫生的重要因素。