Antimicrobial resistance profile of E. coli and Salmonella serovars isolated from diarrheic calves and handlers in Egypt is unknown due to the absence of monitoring. Therefore, this study aimed to determine the virulence, genetic and antimicrobial resistance profiles of E. coli and Salmonella serovars associated with diarrhea in calves and handlers in intensive dairy farms in Egypt. A total of 36 bacterial strains (20 E. coli and 16 Salmonella) were isolated from fecal samples of 80 diarrheic Holstein dairy calves (10 E. coli and 13 Salmonella) and hand swabs of 35 handlers (10 E. coli and 3 Salmonella) in two intensive dairy farms in Sharkia Governate in Egypt. E. coli strains belonged to six different serogroups and O114:K90 was the most prevalent serogroup (30%). However, Salmonella strains were serotyped into four different serogroups and S. Kiel was the most prevalent serotype (50%). Thirteen (65%) E. coli isolates were harbouring either stx2, eaeA and/or astA virulence-associated genes. However, stn and spvC virulence genes were detected in 2 (12.5%) and 4 (25%) of Salmonella isolates, respectively. E. coli isolates showed marked resistance to ampicillin (75%), while Salmonella strains exhibited high resistance to amikacin (100%), gentamicin (93.75%) and tobramycin (87.5%). Results of the present study showed that E. coli and Salmonella serovars isolated from diarrheic calves and handlers in intensive dairy farms in Egypt exhibited resistance to multiple classes of antimicrobials, which may pose a public health hazard. Thus, the continuous monitoring of antimicrobial resistance is necessary for both humans and veterinary medicine to decrease the economic losses caused by antimicrobial-resistant strains in animals as well as the zoonotic risk.

中文翻译：

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从腹泻犊牛和犊牛处理者分离得到的大肠杆菌和沙门氏菌血清素的抗菌素，病毒学和遗传多样性。

由于缺乏监测，从埃及的腹泻小牛和处理者中分离出的大肠杆菌和沙门氏菌血清型的抗菌素耐药性未知。因此，本研究旨在确定埃及集约化奶牛场中犊牛和操作者腹泻相关的大肠杆菌和沙门氏菌血清型的毒力，遗传和抗药性。从80个腹泻的荷斯坦奶牛犊（10个大肠杆菌和13个沙门氏菌）的粪便样本和35个操作员的拭子（10个大肠杆菌和3个沙门氏菌）中分离出总共36个细菌菌株（20个大肠杆菌和16个沙门氏菌）。在埃及Sharkia省的两个集约化奶牛场中。大肠杆菌菌株属于六个不同的血清群，O114：K90是最普遍的血清群（30％）。但是，沙门氏菌菌株被分型为四个不同的血清群和S。基尔是最流行的血清型（50％）。13株（65％）大肠杆菌分离株带有stx2，eaeA和/或astA毒力相关基因。但是，分别在2（12.5％）和4（25％）沙门氏菌分离物中检测到stn和spvC毒力基因。大肠杆菌分离株显示出对氨苄西林的显着耐药性（75％），而沙门氏菌菌株显示出对阿米卡星（100％），庆大霉素（93.75％）和妥布霉素（87.5％）的高耐药性。本研究的结果表明，在埃及的集约化奶牛场中，从腹泻小牛和处理者身上分离出的大肠杆菌和沙门氏菌血清表现出对多种抗菌素的耐药性，这可能构成公共健康危害。从而，