BACKGROUND Many commercially important drug and flavor compounds are secondary metabolites of terpenoid origin. Pogostemon cablin, a commercially important industrial and medicinal crop, accumulates abundant patchouli oil comprised of more than 24 unique sesquiterpene compounds, with the most abundant being patchouli alcohol. RESULTS In this study, we analyzed the P. cablin transcriptome library, obtaining 74 terpenoid biosynthesis-related genes, and identified their expression patterns in leaves, stems, and flowers. These genes are members of 15 different families, and we detected all the enzymes involved in the sesquiterpenes pathway that are responsible for patchoulol biosynthesis. Sequence structure, homology, conserved domain properties, and phylogeny of certain identified genes were systematically investigated. Color complementation assay was used to verify the functional activity of the MEP pathway proteins. Exogenous hormone treatment revealed that patchoulol synthesis is induced by methyl jasmonate (MeJA). Quantitative reverse-transcription PCR analysis indicated that the MVA pathway genes (acetoacetyl-CoA thiolase, 3-hydroxy-3-methylglutaryl-coenzyme A reductase, mevalonate diphosphate decarboxylase, and farnesyl diphosphate synthase) participate in patchoulol biosynthesis and are mediated by MeJA. CONCLUSIONS Taken together, this is the first report of integrated analysis of P. cablin MVA and MEP pathway related genes, providing a better understanding of terpenoid and/or patchoulol biosynthesis in P. cablin, and the basis for improving patchoulol production through genetic engineering.

中文翻译：

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倍半萜通路基因的分子鉴定和表达，其负责广emon香中的广patch香酚的生物合成和调控。

背景技术许多商业上重要的药物和调味剂化合物是萜类化合物的次生代谢产物。Pogostemon cablin是一种商业上重要的工业和药用作物，它积聚了丰富的广patch香油，其中包含超过24种独特的倍半萜烯化合物，其中最多的是广patch香醇。结果在这项研究中，我们分析了卡宾氏杆菌转录组文库，获得了74个与萜类生物合成相关的基因，并鉴定了它们在叶，茎和花中的表达模式。这些基因是15个不同家族的成员，我们检测到了倍半萜途径中所有参与了布图洛尔生物合成的酶。系统地研究了某些已鉴定基因的序列结构，同源性，保守结构域特性和系统发育。颜色互补测定用于验证MEP途径蛋白的功能活性。外源激素治疗表明，茉莉酸甲酯（MeJA）诱导了广patch香酚的合成。定量逆转录PCR分析表明，MVA途径基因（乙酰乙酰辅酶A硫解酶，3-羟基-3-甲基戊二酰辅酶A还原酶，甲羟戊酸二磷酸脱羧酶和法呢基二磷酸合酶）参与了广patch香酚的生物合成，并由MeJA介导。结论综上所述，这是综合分析卡氏假单胞菌MVA和MEP途径相关基因的第一份报告，可更好地了解卡氏假单胞菌中的萜类和/或广bio香酚生物合成，以及通过基因工程改善广patch香酚产量的基础。