BACKGROUND There were few report concerning anti-glycation and antioxidant activities of the minor amounts of components in curcuminoids, demethylcurcumin and tetrahydroxycurcumin, in vitro and in vivo. RESULTS The bovine serum albumin/galactose of non-enzymatic glycation models, radical-induced hemolysis, and oxygen radical absorbance capacity (ORAC) were studied in vitro, and the D-galactose-induced oxidative stress in BALB/c mice and then demethylcurcumin or tetrahydroxycurcumin interventions in vivo. The parameters of oxidative stress in plasma and brain extracts were determined among animal groups with or without both curcuminoids interventions. The demethylcurcumin and tetrahydroxycurcumin exhibited anti-glycation, anti-hemolysis, and ORAC activities, and showed much better and significant difference (P < 0.05) compared to those of curcumin in vitro. In animal experiments, the intervened two curcuminoids at both concentrations showed to lower serum malondialdehyde (MDA), brain MDA levels and iNOS protein expressions, and elevate serum ORAC activities, and showed difference (P < 0.05) compared to the galactose-induced control. CONCLUSION The demethylcurcumin and tetrahydroxycurcumin showed potentials in developing functional foods for antioxidant-related purposes.

中文翻译：

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体外去甲基姜黄素和四羟基姜黄素的抗糖化，抗溶血和ORAC活性，以及​​D-半乳糖诱导的BALB / c小鼠体内氧化应激的降低。

背景技术关于姜黄素，去甲基姜黄素和四羟基姜黄素中微量成分的抗糖基化和抗氧化活性在体外和体内的报道很少。结果研究了非酶促糖基化模型的牛血清白蛋白/半乳糖，自由基诱导的溶血和氧自由基吸收能力（ORAC），然后研究了D-半乳糖诱导的BALB / c小鼠的氧化应激，然后用去甲基姜黄素或四羟基姜黄素的体内干预。在有或没有两种姜黄素干预措施的动物组中确定血浆和脑提取物的氧化应激参数。脱甲基姜黄素和四羟基姜黄素显示出抗糖化，抗溶血和ORAC活性，并显示出更好且显着的差异（P <0。05）与姜黄素的体外比较。在动物实验中，两种浓度的两种姜黄素干预均显示降低了丙二醛（MDA），脑MDA水平和iNOS蛋白表达，并提高了血清ORAC活性，与半乳糖诱导的对照相比有差异（P <0.05）。结论脱甲基姜黄素和四羟基姜黄素在开发与抗氧化剂相关的功能性食品方面具有潜力。