High-mobility group box-1 (HMGB1) is a nuclear protein that promotes inflammation during the acute phase post-stroke, and enhances angiogenesis during the delayed phase. Here, we evaluated whether indirect revascularization surgery with HMGB1 accelerates brain angiogenesis in a chronic cerebral hypoperfusion model. Seven days after hypoperfusion induction, encephalo-myo-synangiosis (EMS) was performed with or without HMGB1 treatment into the temporal muscle. We detected significant increments in cortical vasculature (p < 0.01), vascular endothelial growth factor (VEGF) expression in the temporal muscle (p < 0.05), and ratio of radiation intensity on the operated side compared with the non-operated side after EMS in the HMGB1-treated group than in the control group (p < 0.01). Altogether, HMGB1 with EMS in a chronic hypoperfusion model promoted brain angiogenesis in a VEGF-dependent manner, resulting in cerebral blood flow improvement. This treatment may be an effective therapy for patients with moyamoya disease.

中文翻译：

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慢性脑灌注模型中间接旁路手术后高流动性Box-1诱导的血管生成。

高迁移率族box-1（HMGB1）是一种核蛋白，可在中风后急性期促进炎症，并在延迟期促进血管新生。在这里，我们评估了在慢性脑灌注不足模型中用HMGB1进行的间接血运重建术是否能加速脑血管生成。灌注不足7天后，对颞肌进行HMGB1治疗或不进行HMBO1治疗。 在EMS中，我们检测到 颞肌中皮质脉管系统（p <0.01），血管内皮生长因子（VEGF）表达（p <0.05）以及手术侧与非手术侧的放射强度的显着增加。 HMGB1治疗组比对照组（p <0.01）。总之，在慢性低灌注模型中，带有EMS的HMGB1以VEGF依赖性方式促进了脑血管生成，从而改善了脑血流。这种治疗对于烟雾病患者可能是一种有效的治疗方法。