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Portunus pelagicus mtDNA heteroplasmy inheritance and its effect on the use of mtCR and mtCOI sequence data.
Mitochondrial DNA Part A ( IF 1.695 ) Pub Date : 2019-11-25 , DOI: 10.1080/24701394.2019.1693549
Pavitchaya Koolkarnkhai 1 , Chidchanok Intakham 1 , Pradit Sangthong 1, 2 , Wunrada Surat 1, 2 , Passorn Wonnapinij 1, 2, 3
Affiliation  

Mitochondrial DNA (mtDNA) sequences, especially mitochondrial control region (mtCR) and mitochondrial cytochrome c oxidase subunit I (mtCOI), have been widely used in population and evolutionary genetic analyses of metazoan. The presence of mtDNA heteroplasmy – a mixture of mtDNA haplotypes – possibly affects these analyses. This study aimed to reveal mtDNA heteroplasmy in mtCR, mtCOI, and mtND2 (mitochondrial NADH dehydrogenase subunit 2) of Portunus pelagicus, and examine its effect on the use of mtCR and mtCOI sequences. The screening result showed that the probability of observing mtDNA heteroplasmy was approximately 8%. Across the three targeted regions, 92 heteroplasmic variants were observed from seven samples comprising three mothers and four offspring. Most inherited heteroplasmy presented transition and silence mutation. By comparing the proportion of shared variants among maternal relatives to that among non-relatives, the result suggested that most heteroplasmic variants observed in an individual are inherited. Statistical analyses carried out on the inter-generational differences suggested that random drift and purifying selection play roles in determining the offspring’s heteroplasmy level. The size of the random shift varies according to the location of variants and the mothers. The phylogenetic analysis showed that the presence of mtDNA heteroplasmy in mtCR and mtCOI does not affect familial and species identification, respectively. This study firstly reported the mtDNA heteroplasmy in P. pelagicus, its inheritance pattern, and its effect on the use of mtDNA sequence data. This basic knowledge would be useful for the study based on mtDNA sequence data, especially in other invertebrates.



中文翻译:

远洋马齿线粒体mtDNA异质性遗传及其对mtCR和mtCOI序列数据使用的影响。

线粒体DNA(mtDNA)序列,尤其是线粒体控制区(mtCR)和线粒体细胞色素C氧化酶亚基I(mtCOI),已广泛用于后生动物的种群和进化遗传分析。mtDNA异质性(mtDNA单倍型的混合物)的存在可能会影响这些分析。这项研究的目的是揭示远古Portunus pelagicus mtCRmtCOImtND2(线粒体NADH脱氢酶亚基2)中的mtDNA异质性,并研究其对使用mtCRmtCOI的影响序列。筛选结果表明,观察到mtDNA异质性的可能性约为8%。在三个目标区域中,从包括三个母亲和四个后代的七个样本中观察到92个异质变体。大多数遗传的异质性表现出过渡和沉默突变。通过比较母亲亲属和非亲戚中共有变体的比例,结果表明在个体中观察到的大多数异质变体都是遗传的。对代际差异进行的统计分析表明,随机漂移和纯化选择在确定后代的异质性水平方面发挥了作用。随机移位的大小根据变体和母亲的位置而变化。mtCRmtCOI分别不影响家族鉴定和物种鉴定。这项研究首先报道了P. pelagicus中的mtDNA异质性,其遗传模式及其对mtDNA序列数据使用的影响。这些基础知识对于基于mtDNA序列数据的研究将是有用的,尤其是在其他无脊椎动物中。

更新日期:2019-11-25
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