Vulvovaginal candidiasis (VVC), caused by Candida albicans, affects women’s health and life. We aimed to explore the correlation between ERG3 as well as Efg1 mutation/overexpression and azoles-resistance, and the correlation between ERG3 and Efg1 mRNA expression in C. albicans. First, C. albicans was isolated from clinical VVC patients. ERG3 and Efg1 mutations were detected by polymerase chain reaction (PCR) and sequencing, and the expression levels of these two genes were also identified by qRT-PCR. Correlations between mutation/overexpression of ERG3/Efg1 and azoles-resistance as well as ERG3 and Efg1 mRNA expression were analyzed. Based on the ERG3 sequencing, the results showed that there were 2 missense mutation sites, 1 nonsense mutation site, and 4 silent mutation sites, while 1 missense mutation sites, 1 nonsense mutation site, and 12 silent mutation sites were found in Efg1. Furthermore, the mRNA levels of ERG3 gene in the strains sensitive to FCA, ITR or VRC were higher than those in the strains resistant to FCA, ITR, VRC (P < 0.05). While for the mRNA levels of Efg1, susceptible strains were lower than resistant strains. Besides, there was a significant linear negative correlation between ERG3 and Efg1 mRNA expression (r = − 0.614, P < 0.001).

中文翻译：

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ERG3和Efg1在念珠菌性念珠菌病患者分离的白色念珠菌抗偶氮菌菌株中的调节作用。

由白色念珠菌引起的外阴念珠菌病（VVC）影响妇女的健康和生活。我们旨在探讨白色念珠菌中ERG3以及Efg1突变/过表达与唑类抗性之间的相关性，以及ERG3和Efg1 mRNA表达之间的相关性。首先，从临床VVC患者中分离出白色念珠菌。通过聚合酶链反应（PCR）和测序检测ERG3和Efg1突变，并通过qRT-PCR鉴定这两个基因的表达水平。ERG3 / Efg1的突变/过表达与唑类耐药性以及ERG3的相关性分析Efg1 mRNA的表达。基于ERG3测序，结果显示在Efg1中有2个错义突变位点，1个无义突变位点和4个沉默突变位点，而在Efg1中发现了1个错义突变位点，1个无义突变位点和12个沉默突变位点。此外，对FCA，ITR或VRC敏感的菌株中ERG3基因的mRNA水平高于对FCA，ITR，VRC耐药的菌株（P  <0.05）。对于Efg1的mRNA水平，易感菌株低于抗药性菌株。此外，ERG3和Efg1 mRNA表达之间存在显着的线性负相关（r = − 0.614，P  <0.001）。