The proportion of people suffering from cardiovascular diseases has risen by 34% in the last 15 years in India. Cardiomyopathy is among the many forms of CVD s present. Infection of heart muscles is the suspected etiological agent for the same. Oral pathogens gaining entry into the bloodstream are responsible for such infections. Streptococcus mutans is an oral pathogen with implications in cardiovascular diseases. Previous studies have shown certain strains of S. mutans are found predominantly within atherosclerotic plaques and extirpated valves. To decipher the genetic differences responsible for endothelial cell invasion, we have sequenced the genome of Streptococcus mutans B14. Pan-genome analysis, search for adhesion proteins through a special algorithm, and protein–protein interactions search through HPIDB have been done. Pan-genome analysis of 187 whole genomes, assemblies revealed 6965 genes in total and 918 genes forming the core gene cluster. Adhesion to the endothelial cell is a critical virulence factor distinguishing virulent and non-virulent strains. Overall, 4% of the total proteins in S. mutans B14 were categorized as adhesion proteins. Protein–protein interaction between putative adhesion proteins and Human extracellular matrix components was predicted, revealing novel interactions. A conserved gene catalyzing the synthesis of branched-chain amino acids in S. mutans B14 shows possible interaction with isoforms of cathepsin protein of the ECM. This genome sequence analysis indicates towards other proteins in the S. mutans genome, which might have a specific role to play in host cell interaction.

中文翻译：

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致癌病原体的基因组研究，对心血管疾病有影响。

在过去的15年中，印度患有心血管疾病的人数比例已经上升了34％。心肌病是目前存在的许多形式的CVD之一。怀疑是心肌感染的病因。口腔病原体进入血液是造成此类感染的原因。变形链球菌是一种口腔病原体，与心血管疾病有关。先前的研究表明，某些变形链球菌菌株主要在动脉粥样硬化斑块和脱落的瓣膜中发现。为了破译造成内皮细胞入侵的遗传差异，我们对变形链球菌的基因组进行了测序B14。泛基因组分析，通过特殊算法搜索粘附蛋白，以及通过HPIDB进行蛋白-蛋白相互作用的搜索。对187个完整基因组的泛基因组分析表明，共有9656个基因和918个基因组成了核心基因簇。粘附到内皮细胞是区分有毒和无毒株的关键毒力因子。总体而言，变形链球菌B14中总蛋白的4％被归类为粘附蛋白。预测了假定的粘附蛋白和人类细胞外基质成分之间的蛋白相互作用，揭示了新颖的相互作用。保守的基因，变异链球菌中支链氨基酸的合成B14显示与ECM组织蛋白酶蛋白的同工型的可能相互作用。该基因组序列分析表明，变形链球菌基因组中的其他蛋白质可能在宿主细胞相互作用中起特定作用。