BACKGROUND Representing the first line of host defense against virus infections and an essential link between innate and adaptive immune response, the role of dendritic cells (DCs) in peripheral blood of juvenile-onset recurrent respiratory papillomatosis (JORRP) patients and association with disease activity were still not established. MATERIALS AND METHODS In our present study, 28 JORRP patients and 28 age and sex matched healthy controls were enrolled. The percentage, phenotype and cytokine secretion of DC and was measured by flow cytometry. Plasma cytokine were detected by the enzyme-linked immunosorbent assay (ELISA). RESULTS We found that the percentage of myeloid DC (mDC) was significantly lower in JORRP patients compared to healthy controls and was negatively correlated with interval times, but not surgical times or disease onset. Moreover, the activation marker, CD40 and CD86 was significantly up-regulated on the surfaces of mDC in JORRP patients compared with healthy controls. Neither the percentage nor activation of plasmacytoid DC (pDC) showed statistical difference between JORRP patients and healthy controls. HLA-DR expression on both mDC and pDC was down-regulated in JORRP group and negatively correlated with surgical times. Antigen presenting ability of DC was greatly impaired in JORRP patients of higher number of operations and shorter interval time. Plasma IL-10 as well as IL-10 secreted by mDC was higher in JORRP patients compared with healthy control. Finally, we detected an up-regulated TLR2 and TLR4 expression on mDCs and TLR4 expression was positively correlated with HLA-DR expression on mDC of JORRP patients. CONCLUSION Our results demonstrate an abnormal TLR2 and TLR4 expression in mDCs may contribute to suppressive immune response to HPV6 or HPV11 infection and associated with disease activity in JORRP patients.

中文翻译：

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JORRP患者中的髓样树突状细胞异常激活并与疾病活动相关。

背景代表宿主抵抗病毒感染的第一道防线以及先天性和适应性免疫反应之间的必要联系，树突状细胞（DC）在青少年复发性呼吸道乳头状瘤病（JORRP）患者外周血中的作用以及与疾病活动的关系仍然没有建立。材料与方法在本研究中，我们纳入了28例JORRP患者和28例年龄和性别相匹配的健康对照者。通过流式细胞术测量DC的百分比，表型和细胞因子分泌。通过酶联免疫吸附测定（ELISA）检测血浆细胞因子。结果我们发现，与健康对照组相比，JORRP患者的髓样DC（mDC）百分比显着降低，并且与间隔时间呈负相关，但与手术时间或疾病发作没有负相关。此外，与健康对照组相比，JORRP患者的mDC表面活化标记CD40和CD86明显上调。JORRP患者与健康对照组之间的浆细胞样DC（pDC）百分比或激活均未显示统计学差异。在JORRP组中，mDC和pDC上的HLA-DR表达下调，并且与手术时间负相关。在手术次数较多，间隔时间较短的JORRP患者中，DC的抗原呈递能力大大受损。与健康对照组相比，JORRP患者的血浆IL-10以及mDC分泌的IL-10更高。最后，我们检测到mDCs上TLR2和TLR4表达上调，并且JORRP患者mDCs上TLR4表达与HLA-DR表达正相关。