Present study reports modulation in butanol biosynthesis in Clostridium acetobutylicum ATCC 824 under the influence of zinc supplementation or magnesium starvation either individually or in combination. An improvement in butanol titer from 11.83 g L-1 in control to 13.72 g L-1, 15.79 g L-1, and 19.18 g L-1 was achieved when organism was grown on magnesium starved, zinc supplemented and combined zinc supplemented-magnesium starved fermentation medium, respectively. The elevation in butanol biosynthesis was associated with raised glucose utilization, reduced ethanol production and early induction of solventogenesis. Change in these phenotypic traits of the organism may be attributed to multi-level modulation in central carbon metabolism e.g., upregulation of glycolytic pathway; upregulation in thiolase activity; key intermediate enzyme for biosynthesis of acids and solvent; upregulation in the activity of butyrylaldehyde dehydrogenase & butanol dehydrogenase, the enzymes responsible for butanol biosynthesis and downregulation in alcohol dehydrogenase, redirecting carbon flux from ethanol to butanol.

中文翻译：

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补锌和缺镁对丙酮丁醇梭菌 ATCC 824 中丁醇生物合成的调控

本研究报告了在单独或组合补充锌或镁饥饿的影响下，丙酮丁醇梭菌 ATCC 824 中丁醇生物合成的调节。当生物体在缺镁、补锌和补锌-镁组合上生长时，丁醇滴度从对照的 11.83 g L-1 提高到 13.72 g L-1、15.79 g L-1 和 19.18 g L-1分别是饥饿的发酵培养基。丁醇生物合成的增加与葡萄糖利用增加、乙醇产量减少和溶剂生成的早期诱导有关。生物体这些表型特征的变化可能归因于中央碳代谢的多级调节，例如糖酵解途径的上调；硫解酶活性上调；酸和溶剂生物合成的关键中间体酶；丁醛脱氢酶和丁醇脱氢酶活性的上调，这些酶负责丁醇生物合成和醇脱氢酶的下调，将碳通量从乙醇重定向到丁醇。