The magnetic particle Fe3O4-SiO2-NH2-Cellu-ZIF8 (FSNCZ8) was used to immobilize the high-temperature resistant GH3 β-glucosidase (Tpebgl3) from the Thermomotoga petrophila DSM 13995. The Tpebgl3 has great potential in the catalytic conversion of pharmaceutically active components. The magnetic carrier (FSNCZ8) which provided stronger adsorption capacity, magnetic and high-temperature stability than the previously discussed Fe3O4-NH2-Cellu-ZIF8 without tetraethyl silicate coated. The properties of FSNCZ8-Tpebgl3 (FSNCZ8-T) were as follows: The optimal temperature and pH were 90 °C and 5.5, respectively; the highest activity of FSNCZ8-T approached 2672 U/g; Fe2+ enabled immobilized enzyme to increase its relative activity to 194%. The main characteristics of FSNCZ8-T, including thermal stability, pH stability, and glucose tolerance, were greatly enhanced by adding Fe2+, which was also superior to free enzymes; moreover, the residual activity of FSNCZ8-T was 74% of the initial activity at the end of 10 repeated cycles.

中文翻译：

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耐高温GH3β-葡萄糖苷酶固定在磁性颗粒Fe3O4-SiO2-NH2-Cellu-ZIF8/沸石咪唑酯骨架上

磁性颗粒 Fe3O4-SiO2-NH2-Cellu-ZIF8 (FSNCZ8) 用于固定来自 Thermomotoga petrophila DSM 13995 的耐高温 GH3 β-葡萄糖苷酶 (Tpebgl3)。Tpebgl3 在催化转化药物活性物质方面具有巨大潜力组件。磁性载体 (FSNCZ8) 比之前讨论的没有硅酸四乙酯涂层的 Fe3O4-NH2-Cellu-ZIF8 提供更强的吸附能力、磁性和高温稳定性。FSNCZ8-Tpebgl3（FSNCZ8-T）的性质如下：最适温度和pH分别为90℃和5.5；FSNCZ8-T的最高活性接近2672 U/g；Fe2+ 使固定化酶的相对活性增加到 194%。FSNCZ8-T 的主要特性，包括热稳定性、pH 稳定性和葡萄糖耐受性，添加Fe2+后大大增强，也优于游离酶；此外，FSNCZ8-T 的残留活性在 10 个重复循环结束时为初始活性的 74%。