Dietary restriction (DR) is widely regarded as a viable intervention to extend lifespan and healthspan in diverse organisms. The precise molecular regulatory mechanisms are largely unknown. Epigenetic modifications are not stable upon DR and also keep changing with age. Here, we employed whole genome bisulfite sequencing to determine the DNA methylation changes upon DR in adult Drosophila. Our results indicate that although a low level of DNA methylation exists in the adult Drosophila genome, there is no significant difference in DNA methylation levels upon DR when compared to unrestricted flies. This suggests that other epigenetic components such as histone modifications might be altered by DR.

中文翻译：

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DNA甲基化不参与饮食限制引起的成年果蝇寿命延长。

饮食限制（DR）被广泛认为是延长多种生物寿命和健康寿命的可行干预措施。确切的分子调控机制在很大程度上尚不清楚。表观遗传修饰在DR时不稳定，并且会随着年龄的增长而不断变化。在这里，我们采用了全基因组亚硫酸氢盐测序来确定成年果蝇中DR的DNA甲基化变化。我们的结果表明，尽管成年果蝇基因组中存在低水平的DNA甲基化，但是与不受限制的果蝇相比，DR上的DNA甲基化水平没有显着差异。这表明DR可能会改变其他表观遗传成分，例如组蛋白修饰。