Allergy is a hypersensitivity reaction when exposed to certain environmental substances. It shows high relation between immunoglobulin E (IgE) binding to a specific receptor (FcεRI), pro-inflammatory cytokines, and mediators with allergic inflammation responses. Curcumin is a yellow pigment isolated from the turmeric. Curcumin possesses antioxidant and anti-inflammatory properties as well as exhibits significant chemopreventive activity. This study was aimed to investigate the in vitro assessment of the regulation of curcumin on allergic inflammatory responses on rat basophil leukemia (RBL)-2H3 and human pre-basophils (KU812) cell lines. Curcumin showed the activity against histamine and β-hexosaminidase releases from both IgE-mediated and A23187-induced cells degranulation. The morphological observation also confirmed that curcumin inhibits cells degranulation. IgE-mediated allergic responses and significantly induced mast cells intracellular reactive oxygen species (ROS) production. Curcumin reduced ROS production from IgE-mediated or A23187-induced cells degranulation. Curcumin also successfully reduced FcεRI expressions and some pro-inflammatory cytokines, such as interleukin (IL)-4 and IL-13. Furthermore, curcumin inhibited protein kinase C (PKC)-δ translocation from cytosolic to particulate. These results suggested that curcumin can alleviate both the IgE-mediated and calcium ionosphere A23187-stimulated allergic responses through reducing the release of the allergic mediators.

中文翻译：

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姜黄素通过抑制FcεRI蛋白表达和蛋白激酶Cδ易位对肥大细胞介导的过敏反应的体外抗炎作用。

过敏是指当暴露于某些环境物质中的超敏反应。它显示了与特定受体（FcεRI）结合的免疫球蛋白E（IgE），促炎细胞因子和具有过敏性炎症反应的介质之间的高度关联。姜黄素是一种从姜黄中分离出的黄色颜料。姜黄素具有抗氧化和抗炎特性，并具有显着的化学预防活性。这项研究旨在调查姜黄素对大鼠嗜碱性粒细胞白血病（RBL）-2H3和人类嗜碱性粒细胞（KU812）细胞系过敏性炎症反应的调节的体外评估。姜黄素对IgE介导的和A23187诱导的细胞脱粒均显示出对组胺和β-己糖胺酶释放的活性。形态学观察还证实姜黄素抑制细胞脱粒。IgE介导的过敏反应并显着诱导肥大细胞胞内活性氧（ROS）产生。姜黄素减少了IgE介导的或A23187诱导的细胞脱粒所产生的ROS。姜黄素还成功降低了FcεRI的表达和某些促炎性细胞因子，例如白介素（IL）-4和IL-13。此外，姜黄素抑制蛋白激酶C（PKC）-δ从胞质向颗粒的转运。这些结果表明姜黄素可以通过减少过敏介质的释放来减轻IgE介导的钙离子电离层和钙电离层A23187刺激的过敏反应。IgE介导的过敏反应并显着诱导肥大细胞胞内活性氧（ROS）产生。姜黄素减少了IgE介导的或A23187诱导的细胞脱粒所产生的ROS。姜黄素还成功降低了FcεRI的表达和某些促炎性细胞因子，例如白介素（IL）-4和IL-13。此外，姜黄素抑制蛋白激酶C（PKC）-δ从胞质向颗粒的转运。这些结果表明姜黄素可以通过减少过敏介质的释放来减轻IgE介导的钙离子电离层和钙电离层A23187刺激的过敏反应。IgE介导的过敏反应并显着诱导肥大细胞胞内活性氧（ROS）产生。姜黄素减少了IgE介导的或A23187诱导的细胞脱粒所产生的ROS。姜黄素还成功降低了FcεRI的表达和某些促炎性细胞因子，例如白介素（IL）-4和IL-13。此外，姜黄素抑制蛋白激酶C（PKC）-δ从胞质向颗粒的转运。这些结果表明姜黄素可以通过减少过敏介质的释放来减轻IgE介导的钙离子电离层和钙电离层A23187刺激的过敏反应。此外，姜黄素抑制蛋白激酶C（PKC）-δ从胞质向颗粒的转运。这些结果表明姜黄素可以通过减少过敏介质的释放来减轻IgE介导的钙电离层A23187刺激的过敏反应。此外，姜黄素抑制蛋白激酶C（PKC）-δ从胞质向颗粒的转运。这些结果表明姜黄素可以通过减少过敏介质的释放来减轻IgE介导的钙离子电离层和钙电离层A23187刺激的过敏反应。