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Quantitative Proteomic Map of the Trypanosomatid Strigomonas culicis: The Biological Contribution of its Endosymbiotic Bacterium.
Protist ( IF 2.5 ) Pub Date : 2019-11-01 , DOI: 10.1016/j.protis.2019.125698
Giselle V F Brunoro 1 , Rubem F S Menna-Barreto 2 , Aline S Garcia-Gomes 3 , Carolina Boucinha 4 , Diogo B Lima 5 , Paulo C Carvalho 5 , André Teixeira-Ferreira 1 , Monique R O Trugilho 1 , Jonas Perales 1 , Veit Schwämmle 6 , Marcos Catanho 7 , Ana Tereza R de Vasconcelos 8 , Maria Cristina M Motta 9 , Claudia M d'Avila-Levy 4 , Richard H Valente 1
Affiliation  

Strigomonas culicis is a kinetoplastid parasite of insects that maintains a mutualistic association with an intracellular symbiotic bacterium, which is highly integrated into the protist metabolism: it furnishes essential compounds and divides in synchrony with the eukaryotic nucleus. The protist, conversely, can be cured of the endosymbiont, producing an aposymbiotic cell line, which presents a diminished ability to colonize the insect host. This obligatory association can represent an intermediate step of the evolution towards the formation of an organelle, therefore representing an interesting model to understand the symbiogenesis theory. Here, we used shotgun proteomics to compare the S. culicis endosymbiont-containing and aposymbiotic strains, revealing a total of 11,305 peptides, and up to 2,213 proteins (2,029 and 1,452 for wild type and aposymbiotic, respectively). Gene ontology associated to comparative analysis between both strains revealed that the biological processes most affected by the elimination of the symbiont were the amino acid synthesis, as well as protein synthesis and folding. This large-scale comparison of the protein expression in S. culicis marks a step forward in the comprehension of the role of endosymbiotic bacteria in monoxenous trypanosomatid biology, particularly because trypanosomatids expression is mostly post-transcriptionally regulated.



中文翻译:

锥虫Strigomonas culicis的定量蛋白质组图:其内共生细菌的生物学贡献。

百日咳Strigomonas culicis是一种昆虫的运动质体寄生虫,它与细胞内共生细菌保持相互关联,后者高度整合到原生生物的新陈代谢中:提供必需的化合物并与真核细胞同步分裂。相反,原生生物可被内共生菌治愈,产生共生共生细胞系,这使定居昆虫宿主的能力降低。这种必然的联系可以代表向细胞器形成的进化的中间步骤,因此代表了理解共生理论的有趣模型。在这里,我们使用shot弹枪蛋白质组学来比较S. culicis包含共生共生体和无共生菌的菌株,揭示了总共11,305个肽和多达2,213个蛋白质(野生型和无共生菌分别为2,029和1,452)。与两个菌株之间的比较分析相关的基因本体论表明,受共生体消除影响最大的生物学过程是氨基酸合成,蛋白质合成和折叠。这在蛋白质表达的大规模比较S. culicis标记的步骤在向前内共生细菌在monoxenous锥虫生物学中的作用的理解,特别是因为锥虫表达主要是在转录后调节。

更新日期:2019-11-01
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