Inclusion body hepatitis (IBH), hydropericardium syndrome (HS), and gizzard erosion (GE) are all economically important diseases in the poultry industry worldwide and are all caused by fowl aviadenovirus (FAdV). It is important to identify the serotype of the virus to differentiate these diseases. In the present study, a total of six recent FAdV serotypes were isolated and identified in broiler and broiler-breeder flocks in Izmir, Manisa, and Aydın provinces of the Aegean region of Turkey between January and March 2019. The viruses were isolated from livers and pooled organs of chickens using primary chicken embryo kidney cell cultures (CEKC). Virus isolates were identified by PCR amplification of the loop 1 (L1) variable region of the hexon gene followed by Sanger sequencing. Sequence analysis revealed the presence of both FAdV-D (serotype 11) and FAdV-E (serotype 8b). The viruses that were isolated were associated with IBH, which is typically characterized by gross lesions such as enlarged and pale yellow liver with multiple petechial hemorrhages. Histopathological examination also showed necrotizing hepatitis with intranuclear inclusion bodies in hepatocytes. This study is the first report of the isolation and identification of FAdV serotypes associated with IBH in commercial broilers and broiler-breeder flocks in Turkey. The results of sequence analysis showed that FAdV-8b and FAdV-11 were the circulating serotypes that caused recent field outbreaks of IBH in the Aegean region between January and March, 2019.

中文翻译：

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土耳其商业肉鸡和肉种鸡群中禽类禽腺病毒血清型FAdV-8b和FAdV-11与包涵体肝炎有关。

包涵体肝炎（IBH），心包积水综合征（HS）和胃izz糜烂（GE）在全世界的家禽业中都是重要的经济疾病，都是禽禽腺病毒（FAdV）引起的。识别病毒的血清型以区分这些疾病很重要。在本研究中，2019年1月至3月之间，在土耳其爱琴海地区的伊兹密尔，马尼萨和艾登省的肉鸡和肉种鸡群中共分离并鉴定出六种最近的FAdV血清型。使用原代鸡胚肾细胞培养物（CEKC）合并鸡的器官。通过六邻体基因的loop 1（L1）可变区的PCR扩增，然后进行Sanger测序，鉴定出病毒分离株。序列分析揭示了FAdV-D（血清型11）和FAdV-E（血清型8b）的存在。分离出的病毒与IBH有关，IBH通常以肉眼可见的病变为特征，例如肝脏肿大和淡黄色，伴有多发性出血。组织病理学检查还显示坏死性肝炎，肝细胞内有核内包涵体。这项研究是土耳其商业肉鸡和肉种鸡群中与IBH相关的FAdV血清型分离和鉴定的第一份报告。序列分析结果显示，FAdV-8b和FAdV-11是导致2019年1月至3月之间在爱琴海地区最近爆发IBH的现场血清型。通常以肉眼可见的病变为特征，例如肝脏肿大和淡黄色，伴有多发性出血。组织病理学检查还显示坏死性肝炎，肝细胞内有核内包涵体。这项研究是土耳其商业肉鸡和肉种鸡群中与IBH相关的FAdV血清型分离和鉴定的第一份报告。序列分析结果显示，FAdV-8b和FAdV-11是导致2019年1月至3月之间在爱琴海地区最近爆发IBH的现场血清型。通常以肉眼可见的病变为特征，例如肝脏肿大和淡黄色，伴有多发性出血。组织病理学检查还显示坏死性肝炎，肝细胞内有核内包涵体。这项研究是土耳其商业肉鸡和肉种鸡群中与IBH相关的FAdV血清型分离和鉴定的第一份报告。序列分析结果显示，FAdV-8b和FAdV-11是导致2019年1月至3月之间在爱琴海地区最近爆发IBH的现场血清型。这项研究是土耳其商业肉鸡和肉种鸡群中与IBH相关的FAdV血清型分离和鉴定的第一份报告。序列分析结果显示，FAdV-8b和FAdV-11是导致2019年1月至3月之间在爱琴海地区最近爆发IBH的现场血清型。这项研究是土耳其商业肉鸡和肉种鸡群中与IBH相关的FAdV血清型分离和鉴定的第一份报告。序列分析结果表明，FAdV-8b和FAdV-11是导致2019年1月至3月在爱琴海地区最近爆发IBH的现场血清型。