PURPOSE Circulating adipose stromal cells (CASC) are thought to be increased in obesity and facilitate angiogenesis, and tumor metastases. METHODS CASC were identified from buffy coat peripheral blood mononuclear cells (PBMCs) by flow cytometry as CD34brightCD31- CD45- and CASC frequency was compared to adiposity measures in 33 women at increased risk for breast cancer. Feasibility of CASC as a response biomarker for a diet and exercise intervention in ten breast cancer survivors was then explored. RESULTS For 33 high-risk women, median CASC frequency was 9.7 per million PBMCs and trended positively with body mass index, fat mass index (FMI), and percent android fat. Correlation was significant when BMI was dichotomized at > versus < 35 kg/m2 (p = 0.02). For ten breast cancer survivors with a median BMI of 37 kg/m2, median CASC frequency was 16.4 per million PBMCs. In univariate analyses, change in BMI, total fat and visceral fat were significantly correlated with change in CASC frequency. On multivariate analysis, change in visceral adipose had the strongest association with change in CASC frequency (p < 0.00078). CONCLUSIONS The association between the reduction in visceral adipose tissue and the decrease in frequency of circulating adipose stromal cells suggests that the latter might be a useful biomarker in clinical trials of obese breast cancer survivors undergoing a weight loss intervention.

中文翻译：

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在肥胖乳腺癌幸存者和高危女性的II期能量平衡试验中，循环脂肪基质细胞是一种反应生物标志物。

目的循环脂肪基质细胞（CASC）被认为在肥胖症中增加并促进血管生成和肿瘤转移。方法采用流式细胞术从血沉棕黄层外周血单个核细胞（PBMC）中鉴定出CASC，将33名罹患乳腺癌风险增加的女性的CD34brightCD31-CD45-和CASC频率与肥胖测量进行了比较。然后探讨了CASC作为饮食和运动干预的反应生物标志物在十名乳腺癌幸存者中的可行性。结果对于33名高危女性，CASC的中位数频率为每百万PBMC 9.7，并且与体重指数，脂肪质量指数（FMI）和机器人脂肪百分比呈正相关。当将BMI分为> 35 kg / m2时，相关性显着（p = 0.02）。对于10名BMI中位数为37千克/平方米的乳腺癌幸存者，CASC频率的中位数为每百万PBMC 16.4。在单变量分析中，BMI，总脂肪和内脏脂肪的变化与CASC频率的变化显着相关。在多变量分析中，内脏脂肪的变化与CASC频率的变化具有最强的相关性（p <0.00078）。结论内脏脂肪组织减少与循环脂肪基质细胞频率减少之间的关联表明，后者可能是接受减肥干预的肥胖乳腺癌幸存者临床试验中有用的生物标志物。内脏脂肪的变化与CASC频率的变化有最强的相关性（p <0.00078）。结论内脏脂肪组织减少与循环脂肪基质细胞频率减少之间的关联表明，后者可能是接受减肥干预的肥胖乳腺癌幸存者临床试验中有用的生物标志物。内脏脂肪的变化与CASC频率的变化有最强的相关性（p <0.00078）。结论内脏脂肪组织减少与循环脂肪基质细胞频率减少之间的关联表明，后者可能是接受减肥干预的肥胖乳腺癌幸存者临床试验中有用的生物标志物。