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Exosomes derived from lipopolysaccharide-preconditioned human dental pulp stem cells regulate Schwann cell migration and differentiation
Connective Tissue Research ( IF 2.9 ) Pub Date : 2019-11-26 , DOI: 10.1080/03008207.2019.1694010
Jiabei Li 1 , Yanqin Ju 1 , Shangfeng Liu 1 , Yunyu Fu 1 , Shouliang Zhao 1
Affiliation  

ABSTRACT

Purpose: Schwann cells (SCs) are the main source of odontoblasts. They can migrate to the sites of injury and differentiate into odontoblasts during tooth development and regeneration. However, the molecular mechanisms by which SCs repair dental damage remain to be fully elucidated. In addition, exosomes play a crucial role in regulating cell–cell interaction. Hence, we aim to explore the biological function of exosomes secreted by human dental pulp stem cells (hDPSCs) and their effect on SCs.

Materials and Methods: Exosomes were extracted from the supernatant of hDPSCs (exo) and LPS- preconditioned hDPSCs (LPS-exo), respectively. Following the evaluation of specific surface proteins and exosomes size and morphology, SCs were treated with exo and LPS-exo, and we examined SCs proliferation, migration, and odontogenic differentiation in vitro.

Results: Exosomes had the capacity to regulate SCs proliferation and migration. Furthermore, exosomes from both groups stimulated SCs to produce dentin sialoprotein and undergo mineralization; however, LPS-exo had a better ability to modulate SCs migration and odontogenic differentiation compared with exo.

Conclusions: Exosomes from hDPSCs, especially from LPS- preconditioned hDPSCs, can promote the proliferation, migration and odontogenic differentiation of SCs. LPS might change the hDPSCs’ intercellular signals, which might mediate the odontogenic differentiation of SCs, transmitting in the manner of “exosomes”.



中文翻译:

来自脂多糖预处理的人牙髓干细胞的外泌体调节雪旺细胞迁移和分化

摘要

目的:雪旺细胞(SCs)是成牙本质细胞的主要来源。它们可以迁移到损伤部位并在牙齿发育和再生过程中分化成成牙本质细胞。然而,SCs 修复牙齿损伤的分子机制仍有待充分阐明。此外,外泌体在调节细胞间相互作用中起着至关重要的作用。因此,我们旨在探索人牙髓干细胞 (hDPSCs) 分泌的外泌体的生物学功能及其对 SCs 的影响。

材料和方法:分别从hDPSCs (exo) 和LPS 预处理的hDPSCs (LPS-exo) 的上清液中提取外泌体。在评估特定的表面蛋白和外泌体大小和形态后,用 exo 和 LPS-exo 处理 SCs,我们在体外检测了 SCs 的增殖、迁移和牙源性分化。

结果:外泌体具有调节SCs增殖和迁移的能力。此外,两组的外泌体都刺激 SCs 产生牙本质唾液蛋白并进行矿化。然而,与 exo 相比,LPS-exo 具有更好的调节 SCs 迁移和牙源性分化的能力。

结论:来自 hDPSCs 的外泌体,尤其是来自 LPS 预处理的 hDPSCs,可以促进 SCs 的增殖、迁移和牙源性分化。LPS可能会改变hDPSCs的细胞间信号,从而可能介导SCs的牙源性分化,以“外泌体”的方式传递。

更新日期:2019-11-26
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