Stem cells maintain tissue homeostasis by driving cellular turnover and regeneration upon damage. They reside within specialized niches that provide the signals required for stem cell maintenance. Stem cells have been identified in many tissues and cancer types, but their behavior within the niche and their reaction to microenvironmental signals were inferred from limited static observations. Recent advances in live imaging techniques, such as live cell imaging and intravital microscopy, have allowed the visualization of stem cell behavior and dynamics over time in their (near) native environment. Through these recent technological advances, it is now evident that stem cells are much more dynamic than previously anticipated, resulting in a model in which stemness is a state that can be gained or lost over time. In this review, we will highlight how live imaging and intravital microscopy have unraveled previously unanticipated stem cell dynamics and plasticity during development, homeostasis, regeneration, and tumor formation.

中文翻译：

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使用活体和活细胞显微镜捕获干细胞行为。

干细胞通过在损伤时驱动细胞更新和再生来维持组织稳态。它们位于提供干细胞维持所需信号的专门壁龛中。已在许多组织和癌症类型中鉴定出干细胞，但它们在生态位内的行为及其对微环境信号的反应是从有限的静态观察中推断出来的。活体成像技术的最新进展，例如活细胞成像和活体显微镜，已经允许在其（近）原生环境中随时间推移对干细胞行为和动态进行可视化。通过这些最近的技术进步，现在很明显干细胞比以前预期的更有活力，从而产生了一种模型，其中干细胞是一种可以随着时间而获得或失去的状态。在本次审查中，