当前位置: X-MOL 学术Genetics › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Distinct Elements Confer the Blocking and Bypass Functions of the Bithorax Fab-8 Boundary.
GENETICS ( IF 3.3 ) Pub Date : 2019-11-1 , DOI: 10.1534/genetics.119.302694
Olga Kyrchanova 1 , Daniel Wolle 2 , Marat Sabirov 1 , Amina Kurbidaeva 2 , Tsutomu Aoki 2 , Oksana Maksimenko 1 , Maria Kyrchanova 1 , Pavel Georgiev 1 , Paul Schedl 2, 3
Affiliation  

Boundaries in the Drosophila bithorax complex (BX-C) enable the regulatory domains that drive parasegment-specific expression of the three Hox genes to function autonomously. The four regulatory domains (iab-5, iab-6, iab-7, and iab-8) that control the expression of the Abdominal-B (Abd-B) gene are located downstream of the transcription unit, and are delimited by the Mcp, Fab-6, Fab-7, and Fab-8 boundaries. These boundaries function to block cross talk between neighboring regulatory domains. In addition, three of the boundaries (Fab-6, Fab-7, and Fab-8) must also have bypass activity so that regulatory domains distal to the boundaries can contact the Abd-B promoter. In the studies reported here, we have undertaken a functional dissection of the Fab-8 boundary using a boundary-replacement strategy. Our studies indicate that the Fab-8 boundary has two separable subelements. The distal subelement blocks cross talk, but cannot support bypass. The proximal subelement has only minimal blocking activity but is able to mediate bypass. A large multiprotein complex, the LBC (large boundary complex), binds to sequences in the proximal subelement and contributes to its bypass activity. The same LBC complex has been implicated in the bypass activity of the Fab-7 boundary.

中文翻译:

不同的元素赋予 Bithorax Fab-8 边界的阻塞和旁路功能。

果蝇双胸复合体 (BX-C) 中的边界使驱动三个 Hox 基因的副节段特异性表达的调控域能够自主发挥作用。控制 Abdominal-B (Abd-B) 基因表达的四个调控域(iab-5、iab-6、iab-7 和 iab-8)位于转录单元的下游,并由Mcp、Fab-6、Fab-7 和 Fab-8 边界。这些边界的作用是阻止相邻监管域之间的串扰。此外,三个边界(Fab-6、Fab-7 和 Fab-8)也必须具有旁路活性,以便边界远端的调控域可以接触 Abd-B 启动子。在此报告的研究中,我们使用边界替换策略对 Fab-8 边界进行了功能剖析。我们的研究表明 Fab-8 边界有两个可分离的子元素。远端子元件可阻止串扰,但不能支持旁路。近端子元件仅具有最小的阻断活性,但能够介导旁路。大型多蛋白复合物 LBC(大型边界复合物)与近端子元件中的序列结合并有助于其旁路活性。Fab-7 边界的旁路活动涉及相同的 LBC 复合体。
更新日期:2021-05-08
down
wechat
bug