The aim of the research was to investigate the anti-endotoxin and anti-inflammatory effects of Sinomenine, an agent commonly found in Chinese herbal medicines. Endotoxin (i.e., 1 mg lipopolysaccharide (LPS)/kg)) was administered via intraperitoneal (IP) injection to piglets in high-, middle-, and low-dose sinomenine groups. Piglets were then treated with 1, 5 or 10 mg/kg sinomenine, intramuscularly (i.m.), 3 hr after LPS. Vehicle was administered, as above, to drug control group piglets followed 3 hr later by 10 mg/kg sinomenine i.m.. LPS control group piglets were challenged with 1 mg/kg LPS IP, followed by vehicle i.m., and naïve control piglets were treated with normal saline IP, followed by normal saline i.m., as above. Temperatures were measured, and blood samples were collected from the precaval veins of piglets at 12, 24, and 48 hr post-LPS or vehicle injection. Clinical signs were recorded, and index levels were analyzed via ELISA. Sinomenine was found to reduce the incidence and severity of LPS-induced toxicities, including body temperature elevation, cell adhesion, and systemic inflammation. These data suggest that sinomenine may be effective for regulating inflammatory responses and has the potential for use as an anti-endotoxin therapy.

中文翻译：

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青藤碱在LPS相关疾病中的作用与LBP，Mac-1和L-选择素水平的抑制有关。

该研究的目的是研究中草药中常见的青藤碱的抗内毒素和抗炎作用。通过腹膜内（IP）注射向高，中，低剂量青藤碱组的仔猪施用内毒素（即1 mg脂多糖（LPS）/ kg）。然后在LPS后3小时，肌内（im）用1、5或10 mg / kg青藤碱处理仔猪。如上所述，向药物对照组仔猪施用媒介物，然后在3小时后施用10mg / kg的青藤碱im。用1 mg / kg LPS IP攻击LPS对照组仔猪，然后用媒介物im攻击，对幼稚的对照仔猪先用生理盐水IP，再用生理盐水im处理，如上所述。测量温度，并从12、24，LPS或车辆注射后48小时。记录临床症状，并通过ELISA分析指标水平。发现青藤碱可以降低LPS诱导的毒性的发生率和严重程度，包括体温升高，细胞粘附和全身性炎症。这些数据表明青藤碱可能有效调节炎症反应，并有潜力用作抗内毒素疗法。