BACKGROUND The primary therapeutic strategy in cardiovascular disease is the coronary artery bypass surgery, which in- volves the use of small diameter vascular grafts (<6 mm). Human umbilical arteries could be used as a source for the development of these grafts. OBJECTIVE The aim of this study was the decellularization of human umbilical arteries and the evaluation of their re- cellularization potential. METHODS Decellularization of human umbilical arteries was performed with a detergent based protocol. Histological analysis was performed in order to determine the effect of decellularization. Then, recellularization was performed by using two different approaches. The first approach was the dynamic seeding of human umbilical arteries with Mesenchymal Stromal Cells and the second approach involved the recellularization by using a bioreactor system. RESULTS Histological analysis showed the successful removal of cellular and nuclear materials from the umbilical arteries. In addition, successful recellularization of the vessels was observed with both approaches. CONCLUSION The results of this study indicated that human umbilical arteries could serve as an alternative material for the proper development of small diameter vascular grafts.

中文翻译：

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来自人脐动脉的小直径血管移植物的再细胞化潜力。

背景技术心血管疾病的主要治疗策略是冠状动脉搭桥手术，该技术涉及使用小直径血管移植物（<6 mm）。人体脐动脉可以用作这些移植物发展的来源。目的本研究的目的是对人脐动脉的脱细胞作用及其潜在的再细胞化作用进行评估。方法采用基于去污剂的方案对人脐动脉进行脱细胞。进行组织学分析以确定脱细胞作用。然后，通过使用两种不同的方法进行重新细胞化。第一种方法是用间充质基质细胞动态接种人脐动脉，第二种方法是使用生物反应器系统进行细胞再生。结果组织学分析表明，已成功地从脐动脉中清除了细胞和核材料。另外，两种方法均观察到了血管的成功再细胞化。结论这项研究的结果表明，人的脐动脉可作为小直径血管移植物正常发育的替代材料。