Inflammatory bowel disease (IBD) is a chronic, non-specific, inflammatory gastrointestinal disease that mainly consists of Crohn's disease and ulcerative colitis. However, the aetiology and pathogenesis of IBD are still unclear. B10 (IL-10 producing regulatory B) cells, a subset of regulatory B cells, are known to contribute to intestinal homeostasis and the aberrant frequency of B10 cells is associated with IBD. We have recently reported that B10 cells can be induced by ManLAM (mannose-capped lipoarabinomannan), a major cell-wall lipoglycan of M tb (Mycobacterium tuberculosis). In the current study, the ManLAM-induced B10 cells were adoptively transferred into IL(interleukin)-10-/- mice and the roles of ManLAM-induced B10 cells were investigated in DSS (dextran sodium sulphate)-induced IBD model. ManLAM-induced B10 cells decrease colitis severity in the mice. The B10 cells downregulate Th1 polarization in spleen and MLNs (mesenteric lymph nodes) of DSS-treated mice. These results suggest that IL-10 production by ManLAM-treated B cells contributes to keeping the balance between CD4+ T cell subsets and protect mice from DSS-induced IBD.

中文翻译：

---

甘露糖帽的脂质阿拉伯糖甘露聚糖诱导的B10细胞可降低右旋糖酐硫酸钠诱导的小鼠炎症性肠病的严重程度。

炎性肠病（IBD）是一种慢性，非特异性，炎性胃肠道疾病，主要由克罗恩氏病和溃疡性结肠炎组成。然而，IBD的病因和发病机制仍不清楚。已知B10（产生IL-10的调节性B）细胞是调节性B细胞的子集，有助于肠道的动态平衡，且B10细胞的异常频率与IBD相关。最近，我们报道了B10细胞可以被ManLAM（甘露糖帽的lipoarabinomannan）诱导，Mtb是一种主要的细胞壁脂多糖（结核分枝杆菌）。在当前的研究中，ManLAM诱导的B10细胞被过继转移到IL（interleukin）-10-/-小鼠中，并在DSS（葡聚糖硫酸钠）诱导的IBD模型中研究了ManLAM诱导的B10细胞的作用。ManLAM诱导的B10细胞可降低小鼠结肠炎的严重程度。B10细胞下调了经DSS处理的小鼠的脾脏和MLN（肠系膜淋巴结）中的Th1极化。这些结果表明，经ManLAM处理的B细胞产生IL-10有助于保持CD4 + T细胞亚群之间的平衡，并保护小鼠免受DSS诱导的IBD的侵害。