Aim: The purpose of this study is to examine the protective effects of Dapsone on inflammation of intestinal tissue through inhibition of NF-kB pathway in acetic acid-induced colitis in rats.Methods: Acute colitis was produced by intra-rectal instillation of 2 mL of 4% acetic acid diluted in normal saline. Then, two hours after induction of colitis, DMSO as vehicle, dexamethasone (2 mg/kg) and dapsone (12.5 mg/kg) were given to the animals intraperitoneally (i.p.) and continued for five following days. Evaluation of macroscopic and microscopic damages were done. Myeloid peroxidase enzyme (MPO) activity was measured by a biochemical technique. Moreover, tumor necrosis factor-α (TNF-α) activity was identified by ELISA, and the expression level of pNF-kB protein was evaluated by immunohistochemistry (IHC).Results: Dexamethasone (2 mg/kg) and dapsone (12.5 mg/kg) decreased the macroscopic and microscopic damages compared with acetic acid group (p ˂ .001). Additionally, these agents decreased the activity of MPO (p ˂ .001), TNF-α (p ˂ .001) and the expression level of p-NF-kB (p ˂ .001) in rat colon tissue compared with the acetic acid group.Conclusion: It is proposed that the anti-inflammatory activity of dapsone on acetic acid-induced colitis in rats may involve the inhibition of NF-kB pathway.

中文翻译：

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氨苯砜可通过抑制NF-kB信号通路减少乙酸诱导的大鼠结肠组织的炎症反应。

目的：本研究的目的是通过抑制乙酸诱发的结肠炎大鼠中氨苯砜对肠道组织炎症的保护作用。方法：经直肠内滴注2mL可引起急性结肠炎。在生理盐水中稀释的4％乙酸。然后，在诱发结肠炎后两小时，腹膜内（ip）将DMSO作为载体，地塞米松（2mg / kg）和氨苯砜（12.5mg / kg）给予动物，并持续五天。对宏观和微观损伤进行了评估。骨髓过氧化物酶（MPO）活性是通过生化技术测量的。此外，通过ELISA鉴定了肿瘤坏死因子-α（TNF-α）活性，并通过免疫组化（IHC）评估了pNF-kB蛋白的表达水平。与醋酸组相比，地塞米松（2 mg / kg）和氨苯砜（12.5 mg / kg）减少了宏观和微观损伤（p˂0.001）。此外，与乙酸相比，这些试剂降低了大鼠结肠组织中MPO（p ＜0.001），TNF-α（p ＜0.001）和p-NF-kB的表达水平（0.001）。结论：氨苯砜对大鼠乙酸所致结肠炎的抗炎作用可能与抑制NF-κB通路有关。