Nuclear male sterility (NMS) is a potential characteristic in crop recurrent selection and hybrid breeding. Mapping of nuclear male-sterile genes is key to utilizing NMS. Previously, we discovered a spontaneous soybean (Glycine max [L.] Merr.) male-sterile female-fertile mutant NJS-13H, which was conferred by a single recessive gene, designated ms_NJ. In this study, the ms_NJ was mapped to Chromosome 10 (LG O), and narrowed down between two SSR (simple sequence repeats) markers, BARCSOYSSR_10_794 and BARCSOYSSR_10_819 using three heterozygote-derived segregating populations, i.e., (NJS-13H × NN1138-2)F₂, (NJS-13H × N2899)F₂ and (NJS-13H)SPAG (segregating populations in advanced generations). This region spans approximately 1.32 Mb, where 27 genes were annotated according to the soybean reference genome sequence (Wm82.a2.v1). Among them, four genes were recognized as candidate genes for ms_NJ. Comparing to the physical locations of all the known male-sterile loci, ms_NJ is demonstrated to be a new male-sterile locus. This result may help the utilization and cloning of the gene.

中文翻译：

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大豆中一个新的雄性不育基因msNJ的分子作图[Glycine max（L.）Merr。]。

核雄性不育（NMS）是作物轮回选择和杂交育种的潜在特征。核雄性不育基因的定位是利用NMS的关键。以前，我们发现了自发的大豆（Glycine max [L.] Merr。）雄性不育雌性可育突变体NJS-13H，由一个隐性基因（ms _NJ）赋予。在这项研究中，将ms _NJ定位到10号染色体（LG O），并使用三个杂合子来源的分离群体，即（NJS-13H×NN1138-），将其缩小到两个SSR（简单序列重复）标记BARCSOYSSR_10_794和BARCSOYSSR_10_819之间2）F ₂，（NJS-13H×N2899）F ₂和（NJS-13H）SPAG（隔离高龄人口）。该区域跨越大约1.32 Mb，其中根据大豆参考基因组序列（Wm82.a2.v1）注释了27个基因。其中，四个基因被认为是ms _NJ的候选基因。与所有已知的雄性不育基因座的物理位置相比，ms _NJ被证明是一个新的雄性不育基因座。该结果可以帮助基因的利用和克隆。