The Drosophila transgenic technology and fluorescent protein fusions are powerful tools to analyze protein expression patterns, subcellular localization and protein dynamics. Recently, the Drosophila transgenic technology has been improved by the highly efficient phiC31 site-specific integration system. Many new and improved fluorescent proteins with desirable advantages have been developed. However, the phiC31 system and the newly developed fluorescent proteins have not been systematically applied in Drosophila transgenic vectors. Here, we have constructed a modular toolset of C-terminal fluorescent protein fusion vectors based on phiC31 site-specific integration system for the generation of transgenic Drosophila lines. These cloning vectors contain a variety of fluorescent tags, including blue, cyan, green or red fluorescent proteins, photoactivatable or photoswitchable fluorescent proteins, fluorescent timers, photosensitizers and bimolecular fluorescence complementation tags. These vectors provide a range of transcriptional regulation options including UAST, UASP, UASC, LexAop, QUAS, Ubi, αTub67C and αTub84B promoters, and two screening marker options including white and vermilion gene. The vectors have been tested in vivo and can produce fluorescent chimeric proteins that are functional.

中文翻译：

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果蝇基于phiC31的荧光蛋白标签载体的模块化工具集。

果蝇转基因技术和荧光蛋白融合是分析蛋白表达模式，亚细胞定位和蛋白动力学的强大工具。最近，果蝇转基因技术已经通过高效的phiC31位点特异性整合系统得到了改进。已经开发出许多具有所需优点的新型荧光蛋白。但是，phiC31系统和新开发的荧光蛋白尚未系统地应用于果蝇转基因载体。在这里，我们构建了基于phiC31位点特异性整合系统的C末端荧光蛋白融合载体的模块化工具包，用于产生转基因果蝇品系。这些克隆载体包含多种荧光标签，包括蓝色，青色，绿色或红色荧光蛋白，可光激活或可光切换的荧光蛋白，荧光计时器，光敏剂和双分子荧光互补标签。这些载体提供了一系列转录调控选择，包括UAST，UASP，UASC，LexAop，QUAS，Ubi，αTub67C和αTub84B启动子，以及两个筛选标记选择，包括白色和朱红色基因。载体已经在体内进行了测试，可以产生功能性的荧光嵌合蛋白。