The current study aimed to explore the role of the circular RNA circ-TCF4.85 and its downstream target microRNA-486-5p (miR-486-5p) in hepatocellular carcinoma (HCC) development. Circ-TCF4.85 was detected to be highly expressed in HCC tissues. Next, we found that silencing of circ-TCF4.85 repressed HCC cell proliferation, invasion, and migration, while enhancing apoptosis. In addition, biotin-coupled probe pull-down and miRNA capture assays, as well as fluorescence in situ hybridization, confirmed that circ-TCF4.85 could bind to miR-486-5p. In rescue experiments, miR-486-5p had the potential to eliminate the tumor-suppressive effects of circ-TCF4.85 knockdown in HCC. Moreover, miR-486-5p was shown to target ABCF2 gene, which was positively regulated by circ-TCF4.85. Finally, nude mice subcutaneously injected with si-circ-TCF4.85-transfected HCC cells presented with inhibited xenograft tumor formation in vivo. Taken together, our results reveal that silencing of circ-TCF4.85 suppresses HCC progression via miR-486-5p-targeted inhibition of ABCF2.

中文翻译：

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Circ-TCF4.85 沉默通过 microRNA-486-5p 靶向抑制肝细胞癌中的 ABCF2 来抑制癌症进展。

本研究旨在探索环状 RNA circ-TCF4.85 及其下游靶标 microRNA-486-5p (miR-486-5p) 在肝细胞癌 (HCC) 发展中的作用。检测到 Circ-TCF4.85 在 HCC 组织中高表达。接下来，我们发现 circ-TCF4.85 的沉默抑制了 HCC 细胞的增殖、侵袭和迁移，同时增强了细胞凋亡。此外，生物素偶联探针下拉和 miRNA 捕获测定，以及荧光原位杂交，证实 circ-TCF4.85 可以与 miR-486-5p 结合。在挽救实验中，miR-486-5p 有可能消除 circ-TCF4.85 敲低对 HCC 的肿瘤抑制作用。此外，miR-486-5p 显示靶向 ABCF2 基因，该基因受 circ-TCF4.85 正调控。最后，裸鼠皮下注射si-circ-TCF4。85 转染的 HCC 细胞在体内表现出抑制异种移植肿瘤的形成。综上所述，我们的结果表明，circ-TCF4.85 的沉默通过 miR-486-5p 靶向抑制 ABCF2 来抑制 HCC 进展。