Abstract Biofilm growth is a significant source of contamination in the food industry. Enzymes are considered green countermeasures against biofilm formation in the food industry owing to their biodegradability and low toxicity. In this study, the synergistic effect of enzymes was studied against biofilm cleaning from hard surfaces. A mixed-microbial sample was sourced from a meat packaging line and biofilms were grown under high shear conditions on stainless steel and polyethylene surfaces. A model cleaning-in-place (CIP) parallel-plate flow chamber was used for firstly, the enzymatic cleaning and secondly, a disinfection step. The cleaning effectiveness was evaluated in response to different formulations containing non-foaming commercial surfactants among with amylase, protease and lipase at neutral pH. The formulation combining all three enzymes was the most effective, showing a synergy essential for the deformation of biofilm structure and consequently better disinfection of both material surfaces.

中文翻译：

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酶清洁剂对不同表面生物膜清洗的协同作用

摘要 生物膜的生长是食品工业中重要的污染源。由于酶的生物降解性和低毒性，酶被认为是食品工业中对抗生物膜形成的绿色对策。在这项研究中，研究了酶对硬表面生物膜清洁的协同作用。混合微生物样品来自肉类包装线，生物膜在不锈钢和聚乙烯表面的高剪切条件下生长。模型原位清洗 (CIP) 平行板流动室首先用于酶促清洁，其次用于消毒步骤。在中性 pH 值下，根据含有非泡沫商业表面活性剂（包括淀粉酶、蛋白酶和脂肪酶）的不同配方评估清洁效果。