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CYP26B1 declines postnatally in Sertoli cells independently of androgen action in the mouse testis.
Molecular Reproduction and Development ( IF 2.5 ) Pub Date : 2019-11-22 , DOI: 10.1002/mrd.23302
Nadia Y Edelsztein 1 , Kenichi Kashimada 2 , Helena F Schteingart 1 , Rodolfo A Rey 1, 3
Affiliation  

Meiosis begins at puberty and relies on several factors, including androgens and retinoic acid in the mouse testis. CYP26B1 degrades retinoic acid in the testis during prenatal development preventing meiosis initiation. Given the concurrence of meiotic entry and completion of Sertoli cell maturation in response to androgens at puberty in the mouse, we proposed that CYP26B1 is downregulated by androgens in the Sertoli cell during this period. By immunohistochemistry, we showed that CYP26B1 declines in Sertoli cells after birth. However, luciferase reporter assays and quantitative reverse transcription-polymerase chain reaction performed in the prepubertal mouse Sertoli cell line SMAT1 revealed no changes in Cyp26b1 expression in response to androgen treatment. Furthermore, studies carried out using primary Sertoli cells of 10-day-old mice showed no changes in either Cyp26b1 or CYP26B1 expression in response to androgen treatment. In summary, the hereby reported decline in CYP26B1 expression in Sertoli cells towards pubertal onset does not appear to be caused by a direct inhibitory effect of androgens on Sertoli cells in the mouse.

中文翻译:

CYP26B1在出生后在Sertoli细胞中下降,而与雄性激素在小鼠睾丸中的作用无关。

减数分裂始于青春期,并依赖于多种因素,包括小鼠睾丸中的雄激素和视黄酸。CYP26B1在产前发育过程中降解睾丸中的维甲酸,从而防止减数分裂的发生。鉴于减数分裂进入和同时响应小鼠青春期雄激素的Sertoli细胞成熟的完成,我们提出CYP26B1在此期间被Sertoli细胞中的雄激素下调。通过免疫组织化学,我们表明出生后Sertoli细胞中CYP26B1下降。但是,在青春期前的小鼠支持细胞SMAT1中进行的萤光素酶报告基因检测和定量逆转录聚合酶链反应显示,响应雄激素处理,Cyp26b1表达没有变化。此外,使用10天大的小鼠原代支持细胞进行的研究表明,响应雄激素治疗,Cyp26b1或CYP26B1表达均无变化。综上所述,据此特异报道睾丸支持细胞中CYP26B1的表达向青春期发作的下降似乎不是由雄激素直接抑制小鼠睾丸支持细胞引起的。
更新日期:2019-11-01
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