In the concept of a biohybrid lung, endothelial cells seeded on gas exchange membranes form a non-thrombogenic an anti-inflammatory surface to overcome the lacking hemocompatibility of today's oxygenators during extracorporeal membrane oxygenation. To evaluate this concept, the long-term stability and gas exchange performance of endothelialized RGD-conjugated polydimethylsiloxane (RGD-PDMS) membranes was evaluated. Human umbilical vein endothelial cells (ECs) were cultured on RGD-PDMS in a model system under physiological wall shear stress (WSS) of 0.5 Pa for up to 33 days. Gas exchange performance was tested with three biological replicates under elevated WSS of 2.5 Pa using porcine blood adjusted to venous values following ISO 7199 and blood gas analysis. EC morphology was assessed by immunocytochemistry (n = 3). RGD-PDMS promoted endothelialization and stability of endothelialized membranes was shown for at least 33 days and for a maximal WSS of 2.5 Pa. Short-term exposure to porcine blood did not affect EC integrity. The gas transfer tests provided evidence for the oxygenation and decarboxylation of the blood across endothelialized membranes with a decrease of transfer rates over time that needs to be addressed in further studies with larger sample sizes. Our results demonstrate the general suitability of RGD-PDMS for biohybrid lung applications, which might enable long-term support of patients with chronic lung failure in the future.

中文翻译：

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EndOxy：生物混合肺的内皮化气体交换膜的动态长期评估。

在生物混合型肺的概念中，接种在气体交换膜上的内皮细胞形成非血栓形成的抗炎表面，以克服当今体外膜氧合期间充氧器缺乏血液相容性的问题。为了评估该概念，评估了内皮化的RGD共轭聚二甲基硅氧烷（RGD-PDMS）膜的长期稳定性和气体交换性能。在模型系统中，在0.5 Pa的生理壁剪切应力（WSS）下，将人脐静脉内皮细胞（ECs）在RGD-PDMS上培养长达33天。气体交换性能在2.5 WSS升高的WSS下进行了三个生物学重复测试，使用了经过ISO 7199和血气分析后调整为静脉值的猪血。EC形态学通过免疫细胞化学评估（n = 3）。RGD-PDMS可以促进内皮化和内皮化膜的稳定性至少持续33天，最大WSS为2.5 Pa。短期接触猪血不会影响EC的完整性。气体传输测试提供了通过内皮化膜使血液氧化和脱羧的证据，随着时间的推移传输速率降低，这需要在更大样本量的进一步研究中加以解决。我们的结果表明，RGD-PDMS通常适用于生物混合型肺部应用，这可能会在将来为慢性肺衰竭患者提供长期支持。气体传输测试提供了通过内皮化膜使血液氧化和脱羧的证据，随着时间的推移传输速率降低，这需要在更大样本量的进一步研究中加以解决。我们的结果表明，RGD-PDMS通常适用于生物混合型肺部应用，这可能会在将来为慢性肺衰竭患者提供长期支持。气体传输测试提供了通过内皮化膜使血液氧化和脱羧的证据，随着时间的推移传输速率降低，这需要在更大样本量的进一步研究中加以解决。我们的结果表明，RGD-PDMS通常适用于生物混合型肺部应用，这可能会在将来为慢性肺衰竭患者提供长期支持。