The traditional vaccine strains, such as LaSota, do not completely prevent the shedding of NDV. An ideal vaccine which could not only prevent the clinical signs, but significantly reduce the shedding of NDV is urgently needed for the eradication of ND. In this study, an NDV isolate APMV-1/Chicken/China (SC)/PT3/2016 (hereafter referred as PT3) was identified as a class Ⅰ NDV and a lentogenic strain. The antigenic relationship between PT3 and 3 other NDV strains, including vaccine strain LaSota and 2 prevalent genotype Ⅶd and Ⅵb strains were analyzed. The protective efficacy of PT3 and LaSota against challenge with genotype Ⅶd and Ⅵb strains were assessed. The antigenic analysis result showed that 4 strains belong to the single serotype and the PT3 antiserum exhibited the highest HI titer against 3 other NDV strains. The results of protective efficacy showed that both of LaSota and PT3 could provide 100% survivability for infected chickens. However, PT3 performed better in inducing higher humoral responses and reducing virus shedding than the LaSota strain. Lentogenic strains from Class I NDV appear to be promising vaccine candidates for the control of ND, and allows for the easy discrimination of field NDV and vaccine strains.

传统的疫苗株，例如LaSota，不能完全防止NDV脱落。迫切需要一种理想的疫苗，该疫苗不仅可以预防临床症状，而且可以大大减少NDV的散发。在这项研究中，NDV分离株APMV-1 / Chicken / China（SC）/ PT3 / 2016（以下简称PT3）被鉴定为Ⅰ类NDV和一种慢病毒性菌株。分析了PT3与其他3个NDV毒株（包括疫苗株LaSota）和2个流行基因型Ⅶd和Ⅵb株之间的抗原关系。评估了PT3和LaSota对Ⅶd和Ⅵb基因型菌株攻击的保护作用。抗原分析结果表明，有4个菌株属于单一血清型，而PT3抗血清相对于其他3个NDV菌株表现出最高的HI效价。保护效果的结果表明，LaSota和PT3均可为感染鸡提供100％的存活率。但是，与LaSota株相比，PT3在诱导更高的体液反应和减少病毒脱落方面表现更好。来自I类NDV的慢病毒株似乎是用于控制ND的有前途的疫苗候选物，并允许容易地区分NDV和疫苗株。