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Atomic Force Microscopy as a Powerful Multifunctional Tool for Probing the Behaviors of Single Proteins.
IEEE Transactions on NanoBioscience ( IF 3.9 ) Pub Date : 2019-11-18 , DOI: 10.1109/tnb.2019.2954099
Mi Li , Ning Xi , Yuechao Wang , Lianqing Liu

Proteins play a crucial role in regulating life activities and therefore investigating the behaviors of proteins is of critical significance for understanding the underlying mechanisms guiding the physiological and pathological processes of living organisms. Traditional molecular biochemical methods for protein assays are based on ensemble measurements which reflect the averaged behaviors of the whole molecular populations, veiling the rare activities of small molecular subpopulations. Achievements obtained in the past decades have proved that atomic force microscopy (AFM) is a powerful multifunctional tool for characterizing the behaviors of single proteins at work in aqueous conditions with unprecedented spatiotemporal resolution, which provides novel insights into nanoscopic molecular mechanisms and contributes much to the communities of protein biology. In this article, we review the recent advances in AFM-based single-protein analysis from several facets (including morphological imaging, single-molecule force recognition, mechanical unfolding pathways, and high-speed AFM molecular detection), and provide perspectives for future progression.

中文翻译:

原子力显微镜作为探测单一蛋白质行为的强大多功能工具。

蛋白质在调节生命活动中起着至关重要的作用,因此研究蛋白质的行为对于理解指导生物体生理和病理过程的基本机制具有至关重要的意义。用于蛋白质测定的传统分子生化方法基于整体测量,该测量反映了整个分子种群的平均行为,掩盖了小分子亚群的罕见活动。过去几十年中取得的成就证明,原子力显微镜(AFM)是一种功能强大的多功能工具,用于以前所未有的时空分辨率表征水性条件下单个蛋白质在工作中的行为,它为纳米分子机理提供了新颖的见解,并为蛋白质生物学界做出了巨大贡献。在本文中,我们从几个方面(包括形态成像,单分子力识别,机械展开途径和高速AFM分子检测)回顾了基于AFM的单蛋白分析的最新进展,并为未来的发展提供了前景。
更新日期:2019-11-01
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