Bovine viral diarrhea virus (BVDV) is a common contaminant of Madin-Darby bovine kidney (MDBK) cells as well as fetal calf serum (FCS). It is pathogenic to cattle and regulatory authorities require that veterinary vaccine stocks are free from BVDV. MDBK cells are used in the generation of recombinant lumpy skin disease virus (LSDV) and have been used for the growth of LSDV vaccines. This paper describes how vaccine stocks can be cleared of BVDV by passage through an avian host, nonpermissive to BVDV, but permissive to LSDV. LSDV vaccine stocks were shown to be cleared of BVDV after passage on the chorioallantoic membranes (CAMs) of fertilized 7-day old hens' eggs. Vaccines were passaged a second time on CAMs before being grown in primary lamb testes (LT) cells. Vaccines retained BVDV-negative status after passage on LT cells.

中文翻译：

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通过在受精母鸡卵的绒毛膜上传递，从块状皮肤病病毒（LSDV）疫苗库中去除牛病毒性腹泻病毒（BVDV）。

牛病毒性腹泻病毒（BVDV）是Madin-Darby牛肾（MDBK）细胞以及胎牛血清（FCS）的常见污染物。它对牛有致病性，监管机构要求兽用疫苗原种不含BVDV。MDBK细胞用于重组块状皮肤病病毒（LSDV）的产生，并已用于LSDV疫苗的生长。本文介绍了如何通过禽类宿主清除BVDV的疫苗原种，该宿主不允许BVDV，但允许LSDV。LSDV疫苗库存在经过受精的7天大的鸡蛋的绒毛尿囊膜（CAM）上通过后显示已清除BVDV。疫苗在CAM上第二次传代，然后在原代羔羊睾丸（LT）细胞中生长。LT细胞传代后，疫苗保持BVDV阴性状态。