PURPOSE To describe and compare the stromal bed roughness obtained after laser in situ keratomileusis (LASIK) flap creation using a corneal femtosecond laser platform (iFS 150) and a "dual" femtosecond (FS) laser platform (LenSx). METHODS This ex vivo experimental study was conducted in an animal model using 12 freshly enucleated porcine eyes, six with each femtosecond laser. The standard laser treatment parameters were used for the experiment. After LASIK flap creation, the corneal stromal roughness was assessed using atomic force microscopy (AFM) in contact mode immersed in liquid. In each sample, surface measurements were obtained in 60 regions of six eyes per FS laser in 10 20 × 20-micron areas of the central corneal stroma at 512 × 512-point resolution. The surface roughness was measured and the root-mean-square (RMS) values of the roughness were obtained. RESULTS The mean RMS ± standard deviation values were 430 ± 150 nm for the corneal femtosecond laser platform and 370 ± 100 nm for the dual FS laser platform (P < 0.011). CONCLUSIONS In this experimental study with AFM, we found smoother stromal beds after LASIK flap creation with LenSx compared to iFS 150 kHz. Further studies are needed to understand visual implications of the differences found.

中文翻译：

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在猪眼中iFS 150 kHz和创建LenSx飞秒LASIK皮瓣后的角膜基质粗糙度分析

目的描述和比较角膜飞秒激光平台（iFS 150）和“双”飞秒（FS）激光平台（LenSx）激光原位角膜磨镶术（LASIK）皮瓣制作后获得的基质床粗糙度。方法这项离体实验研究是在动物模型中进行的，其中使用了十二只刚去核的猪眼，每只飞秒激光六只。将标准激光处理参数用于实验。LASIK皮瓣制作完成后，使用原子力显微镜（AFM）以浸没在液体中的接触模式评估角膜基质粗糙度。在每个样品中，在每个FS激光的10个20×20微米区域的角膜基质的10个20×20微米区域中，以512×512点的分辨率获得了60个区域的六只眼的表面测量值。测量表面粗糙度并获得粗糙度的均方根（RMS）值。结果角膜飞秒激光平台的平均RMS±标准偏差值为430±150 nm，双FS激光平台的平均RMS±标准偏差值为370±100 nm（P <0.011）。结论在这项关于AFM的实验研究中，我们发现与iFS 150 kHz相比，使用LenSx制作LASIK皮瓣后的基质床更光滑。需要进一步的研究来理解所发现差异的视觉含义。