Eighty-three wild and domestic carnivores of nine species from Janos Biosphere Reserve (JBR), Mexico, were tested by serologic and molecular assays to determine exposure and infection rates of carnivore protoparvovirus 1. Overall, 50.8% (33/65) of the wild carnivores and 100% (18/18) of the domestic dogs tested were seropositive for Canine protoparvovirus 1 (CPV), while 23% (15/65) of the wild carnivores and 22.2% (4/18) of the domestic dogs were PCR positive for CPV. Phylogenetic analysis confirmed circulation of CVP-2 with residues 426 Asn (CPV2a = 1/19) and 426 Glu (CPV-2c = 18/19) among carnivores in JBR. The prevalence of both PCR positivity and antibodies to CPV varied significantly among wild host species. Of the six identified haplotypes, three were unique to kit foxes (Vulpes macrotis) (the species with higher haplotype richness) and two to striped skunks (Mephitis mephitis). The remaining haplotype was shared among all carnivore species including dogs suggesting non-host specificity and bidirectional and continuous viral transmission cycle in the JBR. The phylogenetic similarity of CPV strains from dogs and wild carnivores and the higher prevalence of CPV in wild carnivores captured near towns relative to those captured far from towns suggest that dogs might be an important source of CPV infection for wild carnivores in the JBR. We provide evidence that cross-species transmission occurs at the domestic–wildlife interface in JBR.

中文翻译：

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食肉动物原细小病毒1在墨西哥西北部的野生动物食肉动物界面中。

通过血清学和分子测定法对墨西哥Janos生物圈保护区（JBR）的9种物种的83种野生和家养食肉动物进行了血清学和分子分析测试，以确定食肉动物原细小病毒1的暴露和感染率。总体而言，野生食肉动物为50.8％（33/65）食肉动物和100％（18/18）的家犬对犬细小病毒1（CPV）呈血清反应阳性，而野生食肉动物的23％（15/65）和22.2％（4/18）的家犬经PCR检测每次观看费用正面。系统发育分析证实，JVP中的食肉动物中带有残基426 Asn（CPV2a = 1/19）和426 Glu（CPV-2c = 18/19）的CVP-2循环。在野生宿主物种中，PCR阳性率和抗CPV抗体的患病率差异显着。在六种已鉴定的单倍型中，三只是狐狸独有的（大嘴猴））（具有较高单倍型丰富度的物种）和两个到条纹的臭鼬（Mephitis mephitis）。其余的单倍型在所有食肉动物物种中都共享，包括表明JBR中非宿主特异性以及双向和连续病毒传播周期的狗。来自狗和野生食肉动物的CPV株在系统发育上的相似性以及在城镇附近捕获的野生食肉动物中CPV的患病率高于远离城镇的捕获，这表明在JBR中，狗可能是野生食肉动物CPV感染的重要来源。我们提供的证据表明，跨物种传播发生在JBR的家庭-野生动物交界处。