Aromatase plays a central role in ovarian differentiation and development in teleosts. In the present study, we identified a cyp19a1a homologue from the ovary of Schizothorax prenanti and analysed its expression at both the mRNA and protein levels. Cyp19a1a of S. prenanti showed high homology with that of other teleosts, especially S. kozlovi. The ovary and testis were the main sites of cyp19a1a expression in S. prenanti, and cyp19a1a transcript levels peaked in the mid-vitellogenic (MVG)-stage ovary and the mid-spermatogenic (MS)-stage testis. Signals of Cyp19a1a immunopositivity were detected in the spermatocytes and follicular cells of cortical alveolar-stage and MVG oocytes but not in spermatogonia or spermatids. Taken together, these findings indicate that Cyp19a1a may play an important role in oocyte vitellogenesis as well as spermatocyte development in S. prenanti.

中文翻译：

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CYP19A1A在裂殖壶菌鱼Schizothorax prenanti中的表征和表达谱。

芳香酶在硬骨鱼的卵巢分化和发育中起着核心作用。在本研究中，我们从裂殖假单胞菌的卵巢中鉴定了一个cyp19a1a同源物，并分析了其在mRNA和蛋白质水平上的表达。prenanti的Cyp19a1a与其他硬骨鱼类，尤其是科氏链球菌具有高度同源性。卵巢和睾丸是s。prenanti中cyp19a1a表达的主要位点，而cyp19a1a转录水平在玻璃体中期（MVG）阶段的卵巢和精子中期（MS）阶段的睾丸中达到峰值。Cyp19a1a免疫阳性信号在皮质肺泡期和MVG卵母细胞的精母细胞和滤泡细胞中检测到，但在精原细胞或精子细胞中未检测到。在一起