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Esterase-mediated spinosad resistance in house flies Musca domestica (Diptera: Muscidae).
Ecotoxicology ( IF 2.460 ) Pub Date : null , DOI: 10.1007/s10646-019-02125-y
Yi Zhang,Mingcheng Guo,Zhuo Ma,Chunmei You,Xiwu Gao,Xueyan Shi

Although esterase-mediated spinosad resistance has been proposed for several insects, the associated molecular mechanism remains poorly understood. In this study, we investigated the mechanism of esterase-based spinosad resistance in house flies using a susceptible strain (SSS) and a spinosad-resistant, near-isogenic line (N-SRS). Combined with the synergistic effect of DEF on spinosad in the N-SRS strain, decreased ali-esterase activity in the spinosad-resistant strain has implicated the involvement of mutant esterase in spinosad resistance in house flies. Examination of the carboxylesterase gene MdαE7 in the two strains revealed that four non-synonymous mutations (Trp251-Leu, Asp273-Glu, Ala365-Val, and Ile396-Val) may be associated with spinosad resistance in house flies. Single nucleotide polymorphism analysis further indicated a strong relationship between these four mutations and spinosad resistance. Moreover, quantitative real-time PCR revealed a female-linked MdαE7 expression pattern in the N-SRS strain, which may contribute to sex-differential spinosad resistance in house flies.
更新日期:2020-01-30

 

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