Background: The role of ADIPOQ gene variants in weight loss after different dietary fat amounts remains unclear. Objective: Our aim was to analyze the effects of ADIPOQ gene polymorphism rs266729 on metabolic changes after two different amounts of dietary fat in two hypocaloric diets. Design: A population of 283 obese patients was recruited in a randomized clinical trial with two diets: Diet HF (high-fat diet: 38% carbohydrates, 24% proteins, and 38% fats) versus Diet LF (low-fat diet: 53% carbohydrates, 20% proteins, and 27% fats). Before and after 3 months, an anthropometric evaluation, an assessment of nutritional intake, and a biochemical analysis were carried out. The variant of the ADIPOQgene was assessed by real-time PCR. Results: Weight loss was similar with both diets in both genotypes (CC vs. CG+GG). After dietary intervention with Diet HF, only subjects with CC genotype showed a significant improvement in insulin levels (–3.3 ± 0.6 vs. –1.8 ± 0.9 mU/L; p = 0.03) and the homeostasis model assessment for insulin resistance (HOMA-IR) (–1.3 ± 0.1 vs. –0.8 ± 0.2 units; p = 0.02). After Diet LF, subjects with CC genotype showed a significant improvement in total cholesterol levels (CC vs. CG+GG) (–15.3 ± 1.4 vs. –6.4 ± 1.3 mg/dL; p = 0.01), LDL cholesterol (–14.6 ± 1.8 vs. –6.4 ± 1.3 mg/dL; p = 0.01), insulin levels (–4.6 ± 1.0 vs. –1.6 ± 0.5 mU/L; p = 0.01), and HOMA-IR (–1.6 ± 0.1 vs. –1.0 ± 0.2 units; p = 0.02). Only subjects with CC genotype showed a significant increase of adiponectin levels after both diets (CC vs. CG+GG): Diet HF (10.6 ± 2.0 vs. 1.8 ± 1.0 ng/dL; p = 0.01) and Diet LF (16.1 ± 2.8 vs. 1.3 ± 1.0 ng/dL: p = 0.03). Conclusion: CC genotype of ADIPOQgene variantrs266729 was associated with a better metabolic response after both diets. Additionally, Diet LF produced a significant improvement in lipid profile in noncarriers of allele G.

中文翻译：

---

脂联素基因变体 rs266729 与两种不同低热量饮食的不同常量营养素分布相互作用

背景：ADIPOQ 基因变异在不同膳食脂肪量后体重减轻中的作用尚不清楚。目的：我们的目的是分析 ADIPOQ 基因多态性 rs266729 对两种低热量饮食中两种不同膳食脂肪量后代谢变化的影响。设计：在一项随机临床试验中招募了 283 名肥胖患者，采用两种饮食：饮食 HF（高脂肪饮食：38% 碳水化合物、24% 蛋白质和 38% 脂肪）与饮食 LF（低脂肪饮食：53 % 碳水化合物、20% 蛋白质和 27% 脂肪）。3个月前后分别进行人体测量学评价、营养摄入评价和生化分析。ADIPOQ 基因的变体通过实时 PCR 进行评估。结果：在两种基因型（CC 与 CG+GG）中，两种饮食的体重减轻相似。在使用饮食 HF 进行饮食干预后，只有具有 CC 基因型的受试者的胰岛素水平（–3.3 ± 0.6 vs. –1.8 ± 0.9 mU/L；p = 0.03）和胰岛素抵抗的稳态模型评估 (HOMA-IR) 显示出显着改善)（–1.3 ± 0.1 与–0.8 ± 0.2 单位；p = 0.02）。饮食 LF 后，具有 CC 基因型的受试者的总胆固醇水平（CC 与 CG+GG）（–15.3 ± 1.4 与 –6.4 ± 1.3 mg/dL；p = 0.01）、低密度脂蛋白胆固醇（–14.6 ± 1.8 与 –6.4 ± 1.3 mg/dL；p = 0.01）、胰岛素水平（–4.6 ± 1.0 与 –1.6 ± 0.5 mU/L；p = 0.01）和 HOMA-IR（–1.6 ± 0.1 与 – 1.0 ± 0.2 单位；p = 0.02)。只有具有 CC 基因型的受试者在两种饮食（CC 与 CG+GG）后显示脂联素水平显着增加：饮食 HF（10.6 ± 2.0 对 1.8 ± 1.0 ng/dL；p = 0.01）和饮食 LF（16.1 ± 2.8）与 1.3 ± 1.0 ng/dL：p = 0.03）。结论：ADIPOQgene变体266729的CC基因型与两种饮食后更好的代谢反应相关。此外，饮食 LF 显着改善了等位基因 G 的非携带者的血脂状况。