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Bacterial cellulose synthesized with apple pomace enhanced by ionic liquid pretreatment.
Preparative Biochemistry & Biotechnology ( IF 2.9 ) Pub Date : 2019-11-20 , DOI: 10.1080/10826068.2019.1692222
Wen Zhang 1 , Jian-Jun Wang 1 , Yuan Gao 1 , Le-Le Zhang 1
Affiliation  

Apple pomace was explored as alternative feedstock for producing bacterial cellulose (BC) by Gluconacetobacter xylinus following a cellulase saccharification performed after pretreatment of 1-allyl-3-methylimidazolium chloride ([AMIM]Cl). The dissolving process of apple pomace cellulose was observed by polarized light microscopy (PLM). As FT-IR and XRD results demonstrated, the IL pretreatment proved to be a physical process and no changes in the crystalline structure occurred during the pretreatment. However, the SEM result showed that more fissures and breakages appeared on the surface of pomace microfibers after IL-pretreating, which increased the contact area with cellulase and improved the enzymatic hydrolysis efficiency. An enhancing effect on the BC yield has been observed, 27% higher yield of BC obtained from hydrolysate as compared to sucrose-based medium indicates efficiency of IL-treated apple pomace to serve as high quality feedstock in BC production.

中文翻译:

通过离子液体预处理增强了苹果渣合成的细菌纤维素。

在对1-烯丙基-3-甲基咪唑鎓氯化物([AMIM] Cl)进行预处理后,进行纤维素酶糖化后,苹果渣被用作木糖葡糖杆菌生产细菌纤维素(BC)的替代原料。苹果果渣纤维素的溶解过程通过偏光显微镜观察。如FT-IR和XRD结果所示,IL预处理被证明是一个物理过程,并且在预处理过程中未发生晶体结构变化。SEM结果表明,IL预处理后,果渣微纤维表面出现更多的裂缝和断裂,增加了与纤维素酶的接触面积,提高了酶水解效率。已经观察到对BC产量的增强作用,
更新日期:2020-04-20
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