Photosynthesis is closely related to the growth of plants. A stable reference gene is fundamental for studies of the molecular mechanism of photosynthesis in Lilium regale. Therefore, it is very important to select a suitable reference gene for qRT-PCR analysis on genes of photosynthetic system, chlorophyll biosynthetic pathway and chloroplast development in Lilium regale. Three kinds of tissues, leaves and bulbs (abnormal leaves) of tissue culture plantlets and cotyledons of seedlings of the wild-type and mutant Lilium regale were selected as materials for qRT-PCR. Six housekeeping genes were selected as candidate genes from transcriptome sequencing data of the wild-type and yellow seedling lethal mutant of Lilium regale. Finally, the expression stability of six candidate reference genes was analyzed using geNorm, NormFinder, and BestKeeper software, the comparative ∆Ct method, and the RefFinder program. The results showed that LrActin2 was the best reference gene for qRT-PCR analysis of photosynthesis-related genes expression in leaves of tissue culture plantlets and seedlings of Lilium regale. This study provided useful data for further research on molecular mechanism of photosynthesis in the Lilium.

中文翻译：

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参考基因的选择用于实时定量PCR分析普通百合中光合作用相关基因的表达。

光合作用与植物的生长密切相关。稳定的参考基因是研究百合叶片光合作用分子机制的基础。因此，选择合适的参考基因对普通百合的光合系统基因，叶绿素生物合成途径和叶绿体发育进行qRT-PCR分析是非常重要的。选择组织培养苗的三种组织，叶片和鳞茎（异常叶片）和野生型和突变型百合的子叶作为qRT-PCR的材料。从帝王百合野生型和黄色幼苗致死突变体的转录组测序数据中选择了六个看家基因作为候选基因。最后，使用geNorm，NormFinder和BestKeeper软件，比较∆Ct方法和RefFinder程序分析了六个候选参考基因的表达稳定性。结果表明，LrActin2是qRT-PCR分析王百合百合组培苗和幼苗光合作用相关基因表达的最佳参考基因。该研究为进一步研究百合光合作用的分子机理提供了有益的数据。