Anti-DNA antibodies are now considered as a universal diagnostic feature for the patients with systemic lupus erythematosus (SLE) but the mechanism(s) involved in the generation of these autoantibodies remains to be investigated. Bisphenol A (BPA) is a synthetic phenol extensively used in the manufacturing of polycarbonated plastics. Upon mixing in the diet, it causes several health hazards. This study was undertaken to investigate the contribution of BPA induced DNA damage in SLE patients. Human DNA was modified by BPA in-vitro and the binding characteristics of SLE circulating immunoglobulin Gs (SLE-IgGs) with BPA damaged DNA (BPA-DNA) were screened and compared with the IgGs from normal healthy humans (NH-IgGs). Immunogenicity of BPA-DNA was determined by immunisation in rabbits. DNA from SLE patients (SLE-DNA) or healthy humans (NH-DNA) were isolated and their binding specificity with rabbit anti-BPA-DNA-IgGs was studied. Treatment of human DNA with BPA caused extensive damaged. Circulating SLE-IgGs showed strong recognition of BPA-DNA. BPA-DNA induced high titre antibodies in rabbits. Rabbit anti-BPA-DNA-IgGs showed strong cross reaction with isolated DNA from SLE patients. In short, we concluded that the structural alterations in DNA by BPA, generate neo-epitopes that may be a factor responsible for the induction of anti-DNA autoantibodies in SLE.

中文翻译：

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双酚A修饰的DNA：系统性红斑狼疮中抗DNA自身抗体的可能的免疫原性刺激。

现在，抗DNA抗体被认为是系统性红斑狼疮（SLE）患者的通用诊断功能，但这些自体抗体产生的机制尚待研究。双酚A（BPA）是一种广泛用于制造聚碳酸酯塑料的合成苯酚。混合饮食会引起多种健康危害。这项研究旨在调查BPA诱导SLE患者DNA损伤的作用。通过BPA体外修饰人类DNA，筛选出SLE循环免疫球蛋白Gs（SLE-IgGs）与BPA受损DNA（BPA-DNA）的结合特征，并与正常健康人的IgG（NH-IgGs）进行比较。BPA-DNA的免疫原性是通过免疫兔来确定的。分离来自SLE患者（SLE-DNA）或健康人（NH-DNA）的DNA，并研究其与兔抗BPA-DNA-IgGs的结合特异性。用BPA处理人类DNA造成广泛的损害。循环中的SLE-IgG表现出对BPA-DNA的强烈识别。BPA-DNA诱导了兔高滴度抗体。兔抗BPA-DNA-IgG与SLE患者分离出的DNA表现出强烈的交叉反应。简而言之，我们得出的结论是，BPA对DNA的结构改变会产生新表位，这可能是导致SLE中抗DNA自身抗体诱导的因素。兔抗BPA-DNA-IgG与SLE患者分离出的DNA表现出强烈的交叉反应。简而言之，我们得出的结论是，BPA对DNA的结构改变会产生新表位，这可能是导致SLE中抗DNA自身抗体诱导的因素。兔抗BPA-DNA-IgG与SLE患者分离出的DNA表现出强烈的交叉反应。简而言之，我们得出的结论是，BPA对DNA的结构改变会产生新表位，这可能是导致SLE中抗DNA自身抗体诱导的因素。