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Dose optimisation of PTEN inhibitor, bpV (HOpic), and SCF for the in-vitro activation of sheep primordial follicles.
Growth Factors ( IF 1.8 ) Pub Date : 2019-10-28 , DOI: 10.1080/08977194.2019.1680661
Samane Adib 1, 2 , Mojtaba Rezazadeh Valojerdi 1, 2 , Mehdi Alikhani 3
Affiliation  

The in-vitro development of primordial follicles is critical for improving mammalian fertility and wildlife conservation. This study aimed to optimise the effective doses of bpV (HOpic) and stem cell factor (SCF) for the in-vitro activation of sheep primordial follicles. To do this, sheep ovarian cortex was treated with bpV (1.5, 15, and 150 μM) and SCF (50 and 100 ng/ml). Follicular count indicated that 15 μM bpV and 100 ng/ml SCF significantly increased normal primary follicles compared to other groups (p < 0.05). Also, a significant downregulation of P53 and PTEN, as well as the increased expression of PI3K was observed. The in-vitro maturation was more pronounced when the fragmented tissues were co-treated with selected doses of bpV and SCF. In conclusion, the combination of 15 μM bpV and 100 ng/ml SCF was the most effective treatment strategy for the activation and survival of primordial follicles in sheep ovarian fragments.

中文翻译:

PTEN抑制剂,bpV(HOpic)和SCF的剂量优化,用于体外激活绵羊原始卵泡。

原始卵泡的体外发育对于改善哺乳动物的繁殖力和野生生物保护至关重要。这项研究旨在优化体外激活绵羊原始卵泡的bpV(HOpic)和干细胞因子(SCF)的有效剂量。为此,用bpV(1.5、15和150μM)和SCF(50和100 ng / ml)处理绵羊卵巢皮质。卵泡计数表明,与其他组相比,15μMbpV和100 ng / ml SCF显着增加了正常初级卵泡(p <0.05)。另外,观察到P53和PTEN的显着下调以及PI3K表达的增加。当将碎裂的组织与选定剂量的bpV和SCF共同处理时,体外成熟更为明显。结论,
更新日期:2019-11-01
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