Aim: Effects of two cosurfactants, n-alkylglycosides with mono- or disaccharide groups – N-nonyl β-D-glucopyranoside (N-Glu) and N-decyl β-D-maltoside (D-Mal) – were studied to the stability in saline solution, interaction with serum albumin, and blood circulation of the lipid nanoemulsion (LNE).

Methods: The LNEs composed of soybean oil, phosphatidylcholine, and sodium palmitate were prepared without (Control-LNE) and with N-Glu or D-Mal (NG-LNE and DM-LNE, respectively).

Results: In saline solution, NG-LNE exhibited a smaller droplet size than Control-LNE, while the size of DM-LNE was significantly increased compared with the other LNEs. The fluorescence resonance energy transfer method showed that the order of albumin interaction was DM-LNE > NG-LNE > Control-LNE. In vivo blood circulation in mice, showed greater fractions of both NG-LNE and DM-LNE remaining in blood over time compared with Control-LNE.

Conclusions: The nature of high stability in saline solution and high affinity for serum albumin led to the prolonged circulation of LNE.

目的： 2个辅助表面活性剂的影响，Ñ与单-或二糖基团-alkylglycosides - ñ -壬基β -D-吡喃葡萄糖苷（N-Glu），并且Ñ癸基β -D-麦芽糖苷（d-MAL） -进行了研究，以稳定在盐溶液中，与血清白蛋白的相互作用以及脂质纳米乳剂（LNE）的血液循环。

方法：制备不含（对照LNE），含N-Glu或D-Mal（分别为NG-LNE和DM-LNE）的大豆油，磷脂酰胆碱和棕榈酸钠组成的LNE。

结果：在盐溶液中，NG-LNE的液滴尺寸小于Control-LNE，而DM-LNE的尺寸与其他LNE相比显着增加。荧光共振能量转移法显示白蛋白相互作用的顺序为DM-LNE> NG-LNE>对照-LNE。与对照LNE相比，小鼠体内的血液循环显示随着时间的流逝，血液中残留的NG-LNE和DM-LNE的比例更高。

结论：盐溶液的高稳定性和对血清白蛋白的高亲和性导致了LNE的延长循环。