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Granulocyte colony-stimulating factor downregulates interferon-gamma receptor expression and stimulates interleukin-6 production in activated human macrophages.
Growth Factors ( IF 1.8 ) Pub Date : 2019-09-19 , DOI: 10.1080/08977194.2019.1662418
V I Seledtsov 1 , V V Malashchenko 1 , M E Meniailo 1 , N D Gazatova 1 , G V Seledtsova 2
Affiliation  

We studied direct effects of human granulocyte colony-stimulating factor (G-CSF) on phenotypical properties of human macrophage cells in vitro. CD14+ monocyte/macrophages (Mc/Mphs) were isolated from blood of healthy donors by positive magnetic separation. G-CSF (0.01-1.0 ng/mL), when added to Mc/Mphs along with lipopolysaccharide (LPS, 1.0 μg/mL), was able to noticeably reduce proportions of CD119 (interferon-γ receptor 1)-positive cells, with no stable effects on CD16 (FcγRIII)+ and СD124 (IL-4 receptor subunit alpha)-positive cells. In addition, G-CSF markedly upregulated IL-6 production by LPS-activated Mph cells, without significantly affecting IL-1β, IL-10 and tumor necrosis factor-α (TNF-α) secretion. Our data suggests that G-CSF could restrain Mph polarization to pro-inflammatory (M1) phenotype, thus potentially supporting pro-regenerative Mph activity with implications for immunotherapeutic interventions.

中文翻译:

粒细胞集落刺激因子下调活化人巨噬细胞中干扰素-γ受体的表达并刺激白细胞介素6的产生。

我们研究了人类粒细胞集落刺激因子(G-CSF)对人类巨噬细胞体外表型特性的直接影响。通过正磁分离从健康供体的血液中分离出CD14 +单核细胞/巨噬细胞(Mc / Mphs)。当将G-CSF(0.01-1.0 ng / mL)与脂多糖(LPS,1.0μg/ mL)一起添加到Mc / Mphs中时,能够显着降低CD119(干扰素-γ受体1)阳性细胞的比例,对CD16(FcγRIII)+和СD124(IL-4受体亚基α)阳性细胞没有稳定作用。此外,G-CSF显着上调了LPS激活的Mph细胞的IL-6产生,而没有显着影响IL-1β,IL-10和肿瘤坏死因子-α(TNF-α)的分泌。我们的数据表明,G-CSF可以将Mph极化抑制为促炎性(M1)表型,
更新日期:2019-11-01
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