An electrospinning technique was used to produce three-dimensional (3D) bioactive glass fibrous scaffolds, in the SiO2-CaO sol-gel system, for wound healing applications. Previously, it was thought that 3D cotton wool-like structures could only be produced from sol-gel when the sol contained calcium nitrate, implying that the Ca2+ and its electronic charge had a significant effect on the structure produced. Here, fibres with a 3D appearance were also electrospun from compositions containing only silica. A polymer binding agent was added to inorganic sol-gel solutions, enabling electrospinning prior to bioactive glass network formation and the polymer was removed by calcination. While the addition of Ca2+ contributes to the 3D morphology, here we show that other factors, such as relative humidity, play an important role in producing the 3D cotton-wool-like macrostructure of the fibres. A human dermal fibroblast cell line (CD-18CO) was exposed to dissolution products of the samples. Cell proliferation and metabolic activity tests were carried out and a VEGF ELISA showed a significant increase in VEGF production in cells exposed to the bioactive glass samples compared to control in DMEM. A novel SiO2-CaO nanofibrous scaffold was created that showed tailorable physical and dissolution properties, the control and composition of these release products are important for directing desirable wound healing interactions.

中文翻译：

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静电纺丝3D生物活性玻璃，可用于伤口愈合。

使用静电纺丝技术在SiO2-CaO溶胶-凝胶系统中生产三维（3D）生物活性玻璃纤维支架，用于伤口愈合应用。以前，人们认为只有当溶胶中包含硝酸钙时，才能从溶胶-凝胶中产生3D棉样结构，这意味着Ca2 +及其电荷对所产生的结构具有重大影响。在此，具有3D外观的纤维也由仅包含二氧化硅的组合物电纺而成。将聚合物粘合剂添加到无机溶胶-凝胶溶液中，从而能够在形成生物活性玻璃网络之前进行静电纺丝，并通过煅烧除去聚合物。尽管添加Ca2 +有助于3D形态，但在这里我们显示了其他因素，例如相对湿度，在产生纤维的3D棉羊毛状宏观结构中起着重要作用。将人类皮肤成纤维细胞系（CD-18CO）暴露于样品的溶解产物。进行了细胞增殖和代谢活性测试，并且与DMEM中的对照相比，暴露于生物活性玻璃样品的细胞中VEGF ELISA显示VEGF的产量显着增加。创建了一种新型的SiO2-CaO纳米纤维支架，该支架具有可调节的物理和溶解特性，这些释放产物的控制和组成对于指导所需的伤口愈合相互作用非常重要。进行了细胞增殖和代谢活性测试，并且与DMEM中的对照相比，暴露于生物活性玻璃样品的细胞中VEGF ELISA显示VEGF的产量显着增加。创建了一种新型的SiO2-CaO纳米纤维支架，该支架具有可调节的物理和溶解特性，这些释放产物的控制和组成对于指导所需的伤口愈合相互作用非常重要。进行了细胞增殖和代谢活性测试，并且与DMEM中的对照相比，暴露于生物活性玻璃样品的细胞中VEGF ELISA显示VEGF的产量显着增加。创建了一种新型的SiO2-CaO纳米纤维支架，该支架具有可调节的物理和溶解特性，这些释放产物的控制和组成对于指导所需的伤口愈合相互作用非常重要。