The aim of the present study was to characterize two gram-negative bacterial strains that were isolated from diseased Atlantic Horse Mackerel Trachurus trachurus in 2017. Based on the results obtained from the biochemical and chemotaxonomic characterization, the isolates were identified as Lacinutrix spp. The highest similarity of the 16S rRNA gene sequences was obtained with the strain L. venerupis CECT 8573T (99.1%), while other species showed similarities of 98% (L. jangbogonensis) and 97% (L. algicola and L. mariniflava). Molecular characterization by repetitive element (REP)-PCR and enterobacterial repetitive intergenic consensus (ERIC)-PCR, as well as proteomic characterization by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS), demonstrated heterogeneity between the strains from the Atlantic Horse Mackerel and the type strain, CECT 8573T . The virulence of one of the isolates for Turbot Scophthalmus maximus, European Sea Bass Dicentrarchus labrax, Senegalese Sole Solea senegalensis, and Rainbow Trout Oncorhynchus mykiss was assessed under experimental conditions. No mortalities were recorded after intraperitoneal injections with high doses of bacteria (1 × 109 CFU/mL). Thus, further studies are necessary to elucidate the impact of this bacterial species as a fish pathogen.

中文翻译：

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从大西洋鲭鱼 Trachurus trachurus 中分离出的 Lacinutrix venerupis 的表型和分子特征

本研究的目的是表征 2017 年从患病的大西洋鲭鱼 Trachurus trachurus 中分离出的两种革兰氏阴性细菌菌株。根据生化和化学分类学表征获得的结果，这些菌株被鉴定为 Lacinutrix spp。16S rRNA 基因序列的最高相似性与菌株 L. venerupis CECT 8573T (99.1%) 相似，而其他物种的相似性为 98% (L. jangbogonensis) 和 97% (L. algicola 和 L. mariniflava)。通过重复元件 (REP)-PCR 和肠细菌重复基因间一致 (ERIC)-PCR 进行分子表征，以及通过基质辅助激光解吸/电离飞行时间质谱 (MALDI-TOF-MS) 进行蛋白质组学表征，证明了来自大西洋鲭鱼的菌株和类型菌株 CECT 8573T 之间的异质性。在实验条件下评估了大菱鲆、欧洲鲈鱼 Dicentrarchus labrax、Senegalese Sole Solea senegalensis 和 Rainbow Trout Oncorhynchus mykiss 分离株之一的毒力。腹腔注射高剂量细菌 (1 × 109 CFU/mL) 后未记录到死亡率。因此，需要进一步研究以阐明这种细菌作为鱼类病原体的影响。腹腔注射高剂量细菌 (1 × 109 CFU/mL) 后未记录到死亡率。因此，需要进一步研究以阐明这种细菌作为鱼类病原体的影响。腹腔注射高剂量细菌 (1 × 109 CFU/mL) 后未记录到死亡率。因此，需要进一步研究以阐明这种细菌作为鱼类病原体的影响。