Library preparation is a crucial step in next-generation sequencing workflows. Key determinants of successful library preparation are the available amount of input DNA and the efficiency of the conversion of this DNA into functional library molecules. While the standard blunt-end ligation protocol for Ion Torrent libraries has a theoretical maximum efficiency of 25%, Y-adapters enable highly efficient library preparation by (i) sticky-end ligation and (ii) rendering both DNA strands functional for sequencing, hence resulting in a theoretical efficiency of up to 100%. Moreover, the generation of adapter dimers is reduced. Therefore, we designed, optimized and validated Y-adapters compatible with Ion Torrent sequencing. These facilitate higher library yields combined with overall high sequencing performance regarding the key characteristics read-length, base quality, and library complexity.

中文翻译：

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使用Y适配器进行离子激流测序的高效文库制备。

文库制备是下一代测序工作流程中的关键步骤。成功制备文库的关键决定因素是可用的输入DNA量以及该DNA转化为功能性文库分子的效率。尽管用于离子洪流文库的标准平末端连接协议具有25％的理论最大效率，但Y适配器可通过（i）粘性末端连接和（ii）使两条DNA链都具有测序功能，从而实现高效的文库制备。导致理论效率高达100％。此外，减少了适配器二聚体的产生。因此，我们设计，优化和验证了与离子激流测序兼容的Y适配器。