当前位置:
X-MOL 学术
›
Cell Tissue Bank.
›
论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Effect of glycerol concentrations and temperatures on epidermal growth factor protein expression in preserved canine amniotic membrane.
Cell and Tissue Banking ( IF 1.5 ) Pub Date : 2019-10-14 , DOI: 10.1007/s10561-019-09793-4 Panrawee Phoomvuthisarn 1 , Gunnaporn Suriyaphol 2 , Nalinee Tuntivanich 1
Cell and Tissue Banking ( IF 1.5 ) Pub Date : 2019-10-14 , DOI: 10.1007/s10561-019-09793-4 Panrawee Phoomvuthisarn 1 , Gunnaporn Suriyaphol 2 , Nalinee Tuntivanich 1
Affiliation
Amniotic membrane has been widely applied as a biological graft in both medical and veterinary practice. In ophthalmology, epidermal growth factor (EGF) in human amniotic membrane (HAM) promotes corneal epithelial cell proliferation and migration, thus it facilitates corneal wound healing. In dogs, with limited cryopreserved HAM availability, different cold glycerol preserving protocols have been developed for the storage canine amniotic membrane (CAM). This study aimed to study protein expression of EGF in CAM preserved with different concentrations of glycerol and storage temperatures, using enzyme-linked immunosorbent assay. CAM preserved in 50% glycerol and 99.5% glycerol and kept at 4 and − 20 °C for 7–30 days were compared. We found that preserving membrane with 50% glycerol at − 20 °C has significantly higher EGF protein expression compared with that at 4 °C (p < 0.05). There was a trend that the storage in 50% glycerol achieved higher EGF protein expression than 99.5% glycerol at both 4 °C and − 20 °C. In conclusion, 50% glycerol at − 20 °C was the best condition to preserve CAM in our study. Therefore, there is likely an alternative method to maintain level of EGF protein expression in preserved CAM.
中文翻译:
甘油浓度和温度对保存的犬羊膜中表皮生长因子蛋白表达的影响。
羊膜已被广泛用作医学和兽医实践中的生物移植物。在眼科方面,人羊膜(HAM)中的表皮生长因子(EGF)促进角膜上皮细胞的增殖和迁移,从而促进角膜伤口的愈合。在犬中,冷冻保存的HAM可用性有限,已开发出用于保存犬羊膜(CAM)的不同的冷甘油保存方案。这项研究旨在通过酶联免疫吸附试验研究在不同甘油浓度和保存温度下保存的CAM中EGF的蛋白表达。比较了在50%甘油和99.5%甘油中保存的CAM,并在4和− 20°C下保存7–30天。p <0.05)。有一个趋势是,在4°C和− 20°C时,与99.5%甘油相比,在50%甘油中的存储实现了更高的EGF蛋白表达。总之,在我们的研究中,在− 20°C时50%甘油是保存CAM的最佳条件。因此,可能存在另一种方法来维持保存的CAM中EGF蛋白的表达水平。
更新日期:2019-10-14
中文翻译:
甘油浓度和温度对保存的犬羊膜中表皮生长因子蛋白表达的影响。
羊膜已被广泛用作医学和兽医实践中的生物移植物。在眼科方面,人羊膜(HAM)中的表皮生长因子(EGF)促进角膜上皮细胞的增殖和迁移,从而促进角膜伤口的愈合。在犬中,冷冻保存的HAM可用性有限,已开发出用于保存犬羊膜(CAM)的不同的冷甘油保存方案。这项研究旨在通过酶联免疫吸附试验研究在不同甘油浓度和保存温度下保存的CAM中EGF的蛋白表达。比较了在50%甘油和99.5%甘油中保存的CAM,并在4和− 20°C下保存7–30天。p <0.05)。有一个趋势是,在4°C和− 20°C时,与99.5%甘油相比,在50%甘油中的存储实现了更高的EGF蛋白表达。总之,在我们的研究中,在− 20°C时50%甘油是保存CAM的最佳条件。因此,可能存在另一种方法来维持保存的CAM中EGF蛋白的表达水平。