The purpose of this paper is to analyze the properties of porcine cartilage type II collagen scaffolds crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxy-succinamide (EDC/NHS) under different conditions. The porous EDC/NHS-crosslinked scaffolds were obtained through a two-step freeze-drying process. To determine the optimal crosslinking condition, we used different solvents and various crosslinking temperatures to prepare the scaffolds. Three crosslinking solutions were prepared with different solvents, photographs were taken with a flash in the darkroom, and light transmission was observed. Type II collagen was crosslinked on a horizontal shaker at a speed of 60 r/min according to the above grouping conditions, and then the structural change of the scaffold in each group was observed. To investigate the swelling ratio and the in vitro degradation of the collagen scaffold, tests were also carried out by immersion of the scaffolds in a PBS solution and digestion in type II collagenase, respectively. The influence of the scaffolds on the proliferation of chondrocytes was assessed by the methyl thiazolyl tetrazolium colorimetric assay. The morphology of the crosslinked scaffolds cocultured with chondrocytes was characterized by a scanning electron microscope. The results proved that 75% alcohol and a crosslinking temperature of 37 °C are recommended. Collagen fibrils are more densely packed after crosslinking with EDC/NHS and have a more uniform structure than that of noncrosslinked ones. The EDC-crosslinked scaffolds possessed excellent mechanical property and biocompatibility.

中文翻译：

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EDC / NHS不同交联条件对II型胶原蛋白支架的影响：体外评估。

本文的目的是分析在不同条件下与1-乙基-3-（3-二甲基氨基丙基）碳二亚胺/ N-羟基琥珀酰胺（EDC / NHS）交联的猪软骨II型胶原蛋白支架的性能。多孔EDC / NHS交联的支架是通过两步冷冻干燥过程获得的。为了确定最佳的交联条件，我们使用了不同的溶剂和不同的交联温度来制备支架。用不同的溶剂制备了三种交联溶液，在暗室中用闪光灯拍照，并观察到光透射。根据上述分组条件，II型胶原在水平摇床上以60r / min的速度交联，然后观察每组支架的结构变化。为了研究胶原蛋白支架的溶胀率和体外降解，还通过将支架浸入PBS溶液中并在II型胶原酶中消化来进行测试。通过甲基噻唑基四唑鎓比色测定法评估支架对软骨细胞增殖的影响。通过扫描电子显微镜表征与软骨细胞共培养的交联支架的形态。结果证明，建议使用75％的酒精和37°C的交联温度。与EDC / NHS交联后，胶原蛋白原纤维更紧密地堆积，并且比未交联的胶原蛋白原纤维具有更均匀的结构。EDC交联的支架具有出色的机械性能和生物相容性。还通过将支架浸入PBS溶液中并在II型胶原酶中消化来进行测试。通过甲基噻唑基四唑鎓比色测定法评估支架对软骨细胞增殖的影响。通过扫描电子显微镜表征与软骨细胞共培养的交联支架的形态。结果证明，建议使用75％的酒精和37°C的交联温度。与EDC / NHS交联后，胶原蛋白原纤维更紧密地堆积，并且比未交联的胶原蛋白原纤维具有更均匀的结构。EDC交联的支架具有出色的机械性能和生物相容性。还通过将支架浸入PBS溶液中并在II型胶原酶中消化来进行测试。通过甲基噻唑基四唑比色测定法评估支架对软骨细胞增殖的影响。用扫描电子显微镜表征与软骨细胞共培养的交联支架的形态。结果证明，建议使用75％的酒精和37°C的交联温度。与EDC / NHS交联后，胶原蛋白原纤维更紧密地堆积，并且比未交联的胶原蛋白原纤维具有更均匀的结构。EDC交联的支架具有出色的机械性能和生物相容性。通过甲基噻唑基四唑鎓比色测定法评估支架对软骨细胞增殖的影响。用扫描电子显微镜表征与软骨细胞共培养的交联支架的形态。结果证明，建议使用75％的酒精和37°C的交联温度。与EDC / NHS交联后，胶原蛋白原纤维更紧密地堆积，并且比未交联的胶原蛋白原纤维具有更均匀的结构。EDC交联的支架具有出色的机械性能和生物相容性。通过甲基噻唑基四唑鎓比色测定法评估支架对软骨细胞增殖的影响。用扫描电子显微镜表征与软骨细胞共培养的交联支架的形态。结果证明，建议使用75％的酒精和37°C的交联温度。与EDC / NHS交联后，胶原蛋白原纤维更紧密地堆积，并且比未交联的胶原蛋白原纤维具有更均匀的结构。EDC交联的支架具有出色的机械性能和生物相容性。结果证明，建议使用75％的酒精和37°C的交联温度。与EDC / NHS交联后，胶原蛋白原纤维更紧密地堆积，并且比未交联的胶原蛋白原纤维具有更均匀的结构。EDC交联的支架具有出色的机械性能和生物相容性。结果证明，建议使用75％的酒精和37°C的交联温度。与EDC / NHS交联后，胶原蛋白原纤维更紧密地堆积，并且比未交联的胶原蛋白原纤维具有更均匀的结构。EDC交联的支架具有出色的机械性能和生物相容性。