Aim: To investigate the impact of various feeding strategies on the growth and productivity of a GS-CHO cell line. Methods: Feed additions were conducted at fixed volumes or linked to a marker such as cell growth or metabolism and added as bolus or near-continuously using the automated feeding module of the micro-Matrix (Applikon). Results: The selected feeding regimens supported maximum viable cell densities of up to 1.9 × 10⁷ cells ml^-1 and final titers of up to 1.13 g l^-1. Differences in growth and titer between feeding strategies were insignificant, with the exception of one feeding strategy. Conclusion: As the more complex feeding strategies did not create an advantage, the selection of a simple feeding strategy such as bolus or continuous addition of feed medium is preferred.

中文翻译：

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朝着微型自动补料分批细胞培养工艺的发展方向发展。

目的： 研究各种饲养策略对GS-CHO细胞系生长和生产力的影响。方法：饲料添加量固定或与诸如细胞生长或代谢的标记物关联，并使用微矩阵（Applikon）的自动喂养模块以大剂量或近乎连续的方式添加。结果：选定的喂养方案支持最大存活细胞密度高达1.9×10 ⁷细胞ml ^-1，最终滴度高达1.13 gl ^-1。除一种喂养策略外，两种喂养策略之间的生长和效价差异不明显。结论： 由于更复杂的饲喂策略没有带来优势，因此选择简单的饲喂策略（例如推注或连续添加饲料培养基）是首选。